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Invitrogen
DARPP-32 Recombinant Rabbit Monoclonal Antibody (5T9G4)
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FIGURE: 1 / 1
DARPP-32 Antibody (MA5-38103) in WB
Western blot analysis of DARPP-32 in extracts of various cell lines. Samples were incubated with DARPP-32 Monoclonal antibody (Product # MA5-38103) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-38103
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5T9G4
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DARPP32 (Q9UD71)
Conjugate
Form
Liquid
Concentration
0.33 mg/mL
Amount
33 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse brain, Rat brain
Immunogen sequence: MDPKDRKKIQ FSVPAPPSQL DPRQVEMIRR RRPTPAMLFR LSEHSSPEEE ASPHQRASGE GHHLKSKRPN PCAYTPPSLK AVQRIAESHL QSISNLNENQ
Target Information
DARPP-32 is a dopamine (DA) and AMP-regugated ~32 kDa phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP-32 is thought to play a critical role in the regugation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac®.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DARPP-32; Dopamine- and cAMP-regulated neuronal phosphoprotein; dopamine- and cAMP-regulated phosphoprotein DARPP-32; FLJ20940; IPPD; neuronal phosphoprotein DARPP-32; OTTHUMP00000164275; OTTHUMP00000164276; PPR1B; protein pho; Protein phosphatase 1 regulatory subunit 1B
Gene Aliases: AU040756; DARPP-32; Darpp32; Ppp1r1b
UniProt ID: (Rat) Q6J4I0, (Mouse) Q60829
Entrez Gene ID: (Rat) 360616, (Mouse) 19049
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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