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Invitrogen
DNA Ligase I Recombinant Rabbit Monoclonal Antibody (7B2)
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FIGURE: 1 / 3
DNA Ligase I Antibody (MA5-38384) in IHC (P)
Immunohistochemistry (Paraffin) analysis of DNA Ligase I in paraffin-embedded human placenta tissue using DNA Ligase I Monoclonal Antibody (Product # MA5-38384) diluted at 1:100. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. ... View More
Product Details
MA5-38384
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
7B2
Immunogen
A synthesized peptide derived from human DNA Ligase I
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
20 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol, 150mM NaCl
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody has been tested in direct-ELISA
Target Information
The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA ligase 1; DNA ligase I; ligase 1; ligase I, DNA, ATP-dependent; MGC117397; MGC130025; Polydeoxyribonucleotide synthase [ATP] 1; unnamed protein product
Gene Aliases: hLig1; IMD96; LIG1; LIGI
UniProt ID: (Human) P18858
Entrez Gene ID: (Human) 3978
DNA replication
lagging strand elongation
DNA repair
base-excision repair
base-excision repair, gap-filling
mismatch repair
DNA recombination
cellular response to DNA damage stimulus
anatomical structure morphogenesis
V(D)J recombination
cell division
DNA biosynthetic process
Okazaki fragment processing involved in mitotic DNA replication
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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