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Invitrogen
SMAD1 Recombinant Rabbit Monoclonal Antibody (SD2090)
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FIGURE: 1 / 6
SMAD1 Antibody (MA5-41184) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of SMAD1 in MCF-7 cells (green) using SMAD1 Recombinant Monoclonal Antibody (Product # MA5-41184). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. The nuclear counter stain is DAPI (blue).
Product Details
MA5-41184
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
SD2090
Immunogen
Synthetic peptide within human SMAD1 aa 221-270.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
TBS, pH 7.4, with 0.05% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: BSP-1; DWFC; hSMAD1; JV4-1; JV5-1; MAD homolog 1; MAD, mothers against decapentaplegic homolog 1; Mad-related protein 1; MADH5; Mothers against decapentaplegic homolog 1; mothers against DPP homolog 1; Mothers-against-DPP-related-1; OTTHUMP00000220456; Sma and Mad homolog; SMAD; SMAD 1; SMAD family member 1; SMAD, mothers against DPP homolog 1; SMAD5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1; unnamed protein product
Gene Aliases: BSP-1; BSP1; JV4-1; JV41; MADH1; MADR1; SMAD1
UniProt ID: (Human) Q15797
Entrez Gene ID: (Human) 4086
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
DEAD/H-box RNA helicase binding
protein kinase binding
ubiquitin protein ligase binding
identical protein binding
sequence-specific DNA binding
metal ion binding
co-SMAD binding
I-SMAD binding
primary miRNA binding
MAPK cascade
ossification
osteoblast differentiation
ureteric bud development
mesodermal cell fate commitment
osteoblast fate commitment
cardiac conduction system development
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
cellular iron ion homeostasis
inflammatory response
signal transduction
transforming growth factor beta receptor signaling pathway
gamete generation
cell proliferation
negative regulation of cell proliferation
anatomical structure morphogenesis
embryonic pattern specification
positive regulation of gene expression
cell differentiation
BMP signaling pathway
midbrain development
hindbrain development
primary miRNA processing
developmental process
homeostatic process
positive regulation of osteoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
stem cell differentiation
negative regulation of muscle cell differentiation
cartilage development
cardiac muscle cell proliferation
bone development
SMAD protein signal transduction
positive regulation of cartilage development
cellular response to growth factor stimulus
transforming growth factor beta receptor superfamily signaling pathway
positive regulation of dendrite development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
positive regulation of sprouting angiogenesis
anti-Mullerian hormone signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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