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Invitrogen
ACSL3 Monoclonal Antibody (A8F6)
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FIGURE: 1 / 2
ACSL3 Antibody (MA5-44924) in WB
Western blot analysis of ACSL3 in Hela cell lysates (10 µg/Lane). Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in ACSL3 Monoclonal antibody (Product # MA5-44924) using a dilution of 1:5,000 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Mouse IgG - HRP secondary antibody at a dilution of 1:100,000 for 1 hour at room temperature. Predicted band size: 80 kDa. Observed band size: 70 kDa.
Product Details
MA5-44924
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
A8F6
Immunogen
Recombinant protein within human ACSL3 aa 180-380.
Conjugate
Form
Liquid
Concentration
2.0 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 40% glycerol, 0.1% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
An initial reaction in fatty acid metabolism in eukaryotic cells is activation of fatty acids catalyzed by acyl-CoA synthetase. FACL3 (fatty acid CoA ligase, long-chain 3) is identified as member of the acyl-CoA synthetase (ACS) family by PCR of rat brain cDNAs using primers based on the conserved region of the ACS protein. The 720-amino acid rat protein preferentially utilizes myristate, laurate, arachidonate, and eicosapentaenoate, and is expressed primarily in brain. The predicted 720-amino acid FACL3 human protein is 92% identical to that of rat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Arachidonate--CoA ligase; Fatty acid CoA ligase Acsl3; fatty-acid-Coenzyme A ligase, long-chain 3; LACS 3; lignoceroyl-CoA synthase; Long-chain acyl-CoA synthetase 3; Long-chain-fatty-acid--CoA ligase 3; Medium-chain acyl-CoA ligase Acsl3; predicted protein of HQ2194; unnamed protein product
Gene Aliases: ACS3; ACSL3; FACL3; LACS 3; LACS3; PRO2194
UniProt ID: (Human) O95573
Entrez Gene ID: (Human) 2181
long-chain fatty acid metabolic process
lipid metabolic process
fatty acid metabolic process
neuron differentiation
very-low-density lipoprotein particle assembly
long-chain fatty-acyl-CoA metabolic process
long-chain fatty-acyl-CoA biosynthetic process
positive regulation of Golgi to plasma membrane protein transport
long-chain fatty acid import
positive regulation of secretion
positive regulation of phosphatidylcholine biosynthetic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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