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Ubiquitin Monoclonal Antibody (6C11-B3), APC

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Ubiquitin Monoclonal Antibody (6C11-B3), APC

Product Details

MA5-45230

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunocytochemistry (ICC/IF)

1:100
-

ELISA (ELISA)

Assay-dependent
-
Product Specifications

Species Reactivity

All, Bovine, Human, Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

6C11-B3

Immunogen

Native bovine ubiquitin, conjugated to KLH
3D Epitope / Immunogen

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Amount

200 µg

Purification

Protein G

Storage buffer

95.64mM phosphate/2.48mM MES, pH 7.4, with 0.5M EDTA

Contains

no preservative

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2931684

Product Specific Information

1 µg/mL of MA5-45230 was sufficient for detection of ubiquitin in 10 µg of Hela Lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 10kDa.

Target Information

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 40S ribosomal protein S27-like; 40S ribosomal protein S27-like protein; 40S ribosomal protein S27a; 60S ribosomal protein L40; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin; polyubiquitin B; polyubiquitin C; Polyubiquitin-B; Polyubiquitin-C; pro-ubiquitin; Ribosomal protein eS27-like; ribosomal protein S27; RPL40; RPS27A; rps27l; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; ubiquitin; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein L40; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin precursor; ubiquitin-40S ribosomal protein S27a; ubiquitin-40S ribosomal protein S27a-like; ubiquitin-52 amino acid fusion protein; ubiquitin-60S ribosomal protein L40; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein; ubiquitin/60S ribosomal fusion protein; unnamed protein product

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Gene Aliases: 0610006J14Rik; 1810034D23Rik; 2700054O04Rik; AI194771; AL033289; CEP52; CEP80; D8Ertd21e; eS31; Gm1863; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; TI-225; UBA52; UBA80; UBB; Ubb2; UBC; UBCEP1; UBCEP2; UBCEP80

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UniProt ID: (Rat) P62982, (Bovine) P62992, (Human) P62979, (Bovine) Q3T0B7, (Human) Q71UM5, (Mouse) P62983, (Rat) P24051, (Mouse) Q6ZWY3, (Human) P62987, (Bovine) P63048, (Bovine) P0CG53, (Human) P0CG47, (Human) P0CG48, (Bovine) P0CH28, (Rat) P62986, (Mouse) P62984, (Rat) P0CG51, (Mouse) P0CG49, (Mouse) P0CG50, (Rat) Q63429

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Entrez Gene ID: (Rat) 100912032, (Bovine) 286839, (Human) 6233, (Bovine) 614220, (Human) 51065, (Mouse) 78294, (Rat) 681429, (Mouse) 67941, (Human) 7311, (Bovine) 615199, (Bovine) 281370, (Human) 7314, (Human) 7316, (Bovine) 444874, (Rat) 64156, (Mouse) 22186, (Rat) 192255, (Mouse) 22187, (Mouse) 22190, (Rat) 50522

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Function(s)
structural constituent of ribosome metal ion binding RNA binding protein binding zinc ion binding protein tag ubiquitin protein ligase binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process poly(A) RNA binding protease binding ribosomal protein translational protein
Process(es)
translation cytoplasmic translation protein ubiquitination modification-dependent protein catabolic process ribosomal small subunit biogenesis activation of cysteine-type endopeptidase activity involved in apoptotic process DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation ribosomal small subunit assembly DNA damage response, signal transduction by p53 class mediator biological_process DNA repair cellular response to DNA damage stimulus response to insecticide protein modification process male meiosis I female meiosis I female gonad development hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination male gonad development positive regulation of protein ubiquitination regulation of neuron apoptotic process adipose tissue development ubiquitin homeostasis ubiquitin-dependent protein catabolic process
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