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Invitrogen
GAD65 Recombinant Rabbit Monoclonal Antibody (HL1187)
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FIGURE: 1 / 9
GAD65 Antibody (MA5-46952) in ICC/IF
Immunofluorescent analysis of GAD65 in DIV10 rat E18 primary cortical neuron cells. Cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GAD65 stained by GAD65 monoclonal antibody (Product # MA5-46952) diluted at 1:500. Red: Tau, a Tau marker, stained by Tau antibody [GT287] diluted at 1:500. Blue: Fluoroshield with DAPI.
Product Details
MA5-46952
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Cat,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
HL1187
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GAD65. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Store as concentrated solution.
Centrifuge briefly prior to opening vial.
Target Information
GAD65 (glutamic acid decarboxylase-65) are present in autoimmune disorders such as insulin-dependent (type1) diabetes mellitus (IDDM), stiff man syndrome and polyendocrine autoimmune disease. Auto-antibodies to GAD65 are present in 60-70% of individuals with newly diagnosed IDDM and thus are important markers for disease activity. These auto-antibodies usually recognize conformation-dependent regions on GAD65 and rarely bind to the second isoform, GAD67. Auto-antibodies to GAD67 are found in only 15% of recent-onset IDDM patients and most of this binding can be blocked with GAD65, suggesting shared epitopes between the two isoforms of GAD.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 65 kDa glutamic acid decarboxylase; 65kDa; pancreatic beta cell autoantigen; GAD-65; Glutamate decarboxylase 2; Glutamate decarboxylase 2 (islet); glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa); Glutamate decarboxylase 65 kDa isoform; Glutamate decarboxylase-2 (pancreas); glutamic acid decarboxylase 2; glutamic acid decarboxylase 65; MGC161605; MGC161607; RP11-420F12.2; unnamed protein product
Gene Aliases: 6330404F12Rik; GAD(65); Gad-2; GAD2; GAD65; zgc:112198
UniProt ID: (Dog) Q4PRC2, (Human) Q05329, (Mouse) P48320, (Rat) Q05683
Entrez Gene ID: (Dog) 487107, (Human) 2572, (Mouse) 14417, (Rat) 24380, (Zebrafish) 550403
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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