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Invitrogen
MAPRE3 Monoclonal Antibody (KT53)
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FIGURE: 1 / 1
MAPRE3 Antibody (MA5-47714) in WB
Western blot of MAPRE3 in mouse brain (lanes 1-5). The blot was probed with rat EB1 monoclonal antibody (Product # MA5-47663) (lane 1), EB1/EB2/EB3 monoclonal antibody (Product # MA5-47713) (lane 2), MAPRE2 monoclonal antibody (Product # MA5-47664) (lane 3), MAPRE3 monoclonal antibody (Product # MA5-47714) (lane 4), and anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.
Product Details
MA5-47714
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rat
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
KT53
Immunogen
Clone KT53 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3.
Conjugate
Form
Liquid
Concentration
~1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody detects a 32 kDa* prtoein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.
Target Information
EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APC binding protein; EB1 protein family member 3; EB3; EBF3; End-binding protein 3; MAPRE3; Microtubule-associated protein RP/EB family member 3; RP3; unnamed protein product
Gene Aliases: AI790651; EB2; EB3; EBF3; EBF3-S; MAPRE3; RP3
UniProt ID: (Human) Q9UPY8, (Mouse) Q6PER3, (Rat) Q5XIT1
Entrez Gene ID: (Human) 22924, (Mouse) 100732, (Rat) 298848
protein localization
regulation of microtubule polymerization or depolymerization
regulation of microtubule polymerization
protein localization to microtubule
positive regulation of transcription, DNA-templated
behavioral response to cocaine
spindle assembly
cell division
positive regulation of dendritic spine morphogenesis
cell cycle
mitotic nuclear division
positive regulation of cyclin-dependent protein serine/threonine kinase activity
positive regulation of microtubule plus-end binding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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