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Invitrogen
CRBN Recombinant Rabbit Monoclonal Antibody (PSH0-69)
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FIGURE: 1 / 6
CRBN Antibody (MA5-49359) in IHC (P)
Immunohistochemical analysis of CRBN on formalin-fixed paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissue was blocked in 1% BSA for 20 minutes at room temperature, then probed with CRBN Rabbit Recombinant Monoclonal Antibody (PSH0-69) (Product # MA5-49359) at 1:200 dilution for 1 hour at room ... View More
Product Details
MA5-49359
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-69
Immunogen
Recombinant protein within human crbn aa 1-442 / 442.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Sequence Similarities: 95% Mouse/Rat.
Tissue Specificity: Widely expressed. Highly expressed in brain.
Positive Control: HEK-293 cell lysate, A375 cell lysate, A549 cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, NIH/3T3 cell lysate, MCF7 cell lysate, mouse brain tissue lysate, rat testis tissue lysate, human testis tissue, human brain tissue.
Subcellular Location: Cytoplasm, Nucleus, Membrane.
Predicted band size: 51 kDa.
Target Information
CRBN, a member of the Lon protease protein family, plays a role in brain development. It is a component of the DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. CRBN is thought to regulate the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. It is widely expressed and highly expressed in brain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AD-006; DKFZp781K0715; MGC27358; novel lethal; Protein cereblon; Protein PiL; protein x 0001; unnamed protein product
Gene Aliases: 2610203G15Rik; 2900045O07Rik; AD-006; AF229032; AW108261; CRBN; MRT2; MRT2A; piL
UniProt ID: (Human) Q96SW2, (Mouse) Q8C7D2, (Rat) Q56AP7
Entrez Gene ID: (Human) 51185, (Mouse) 58799, (Rat) 297498
protein ubiquitination
positive regulation of Wnt signaling pathway
negative regulation of protein complex assembly
positive regulation of protein complex assembly
negative regulation of ion transmembrane transport
locomotory exploration behavior
proteasome-mediated ubiquitin-dependent protein catabolic process
limb development
proteolysis
negative regulation of protein homooligomerization
positive regulation of protein homodimerization activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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