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Invitrogen
WNT8A Recombinant Rabbit Monoclonal Antibody (PSH0-39)
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FIGURE: 1 / 4
WNT8A Antibody (MA5-50826) in ICC/IF
Immunocytochemistry analysis of WNT8A in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with WNT8A recombinant monoclonal antibody (Product # MA5-50826) with a dilution of 1:50 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. Nuclear DNA was lab... View More
Product Details
MA5-50826
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH0-39
Immunogen
Recombinant protein within human WNT8A aa 100-300/351.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, Jurkat cell lysate, Raji cell lysate, NIH/3T3 cell lysate, mouse brain tissue lysate, mouse embryo tissue lysate, rat brain tissue lysate, NIH/3T3, HeLa.
Predicted band size: 38.8 kDa
Subcellular Location: Secreted, extracellular space, extracellular matrix.
Target Information
WNT8A, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Protein Wnt-8a; Protein Wnt-8d; Stimulated by retinoic acid gene 11 protein; wingless-related MMTV integration site 8A; wingless-related MMTV integration site 8D; wingless-type MMTV integration site family, member 8A; wnt-8; WNT8d
Gene Aliases: Stra11; Wnt-8A; Wnt-8D; WNT8A; WNT8D
UniProt ID: (Human) Q9H1J5, (Mouse) Q64527
Entrez Gene ID: (Human) 7478, (Mouse) 20890, (Rat) 291678
anterior/posterior axis specification
dorsal/ventral axis specification
neural crest cell fate commitment
Wnt signaling pathway
neuron differentiation
response to retinoic acid
cell fate commitment
positive regulation of transcription from RNA polymerase II promoter
canonical Wnt signaling pathway
secondary palate development
cell morphogenesis
regionalization
multicellular organism development
endoderm development
polarity specification of anterior/posterior axis
polarity specification of proximal/distal axis
regulation of protein localization
establishment of organ orientation
palate development
biological_process
canonical Wnt signaling pathway involved in cardiac muscle cell fate commitment
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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