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Invitrogen
RNF8 Recombinant Rabbit Monoclonal Antibody (8M7D8)
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FIGURE: 1 / 4
RNF8 Antibody (MA5-51454) in IHC (P)
Immunohistochemistry analysis of RNF8 in paraffin-embedded human testis. Samples were incubated with RNF8 Recombinant monoclonal antibody (Product # MA5-51454) using a dilution of 1:300 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Product Details
MA5-51454
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8M7D8
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.215 mg/mL
Amount
21.5 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MGEPGFFVTG DRAGGRSWCL RRVGMSAGWL LLEDGCEVTV GRGFGVTYQL VSKICPLMIS RNHCVLKQNP EGQWTIMDNK SLNGVWLNRA RLEPLRVYSI HQGDYIQLGV PLENKENAEY EYEVTEEDWE TIYPCLSPKN DQMIEKNKEL RTKRKFSLDE LAGPGAEGPS NLKSKINKVS CESGQPVKSQ GKGEVASTPS DNLDPKLTAL EPSKTTGAPI YPGFPKVTEV HHEQKASNSS ASQRSLQMFK VTMSRILRLK
Target Information
The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ActA binding protein 2; ActA-interacting protein 37; AIP37; C3HC4-type zinc finger protein; E3 ubiquitin-protein ligase RNF8; FLJ12013; hRNF8; LaXp180; OTTHUMP00000039684; ring finger protein (C3HC4 type) 8; RING finger protein 8; ring finger protein 8, E3 ubiquitin protein ligase; RING-type E3 ubiquitin transferase RNF8; UBC13/UEV-interacting ring finger protein; unnamed protein product; UURF1
Gene Aliases: 3830404E21Rik; AIP37; hRNF8; KIAA0646; RNF8
UniProt ID: (Human) O76064, (Rat) Q4KLN8, (Mouse) Q8VC56
Entrez Gene ID: (Human) 9025, (Rat) 361815, (Mouse) 58230
chromatin binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
ubiquitin protein ligase binding
histone binding
identical protein binding
protein homodimerization activity
ubiquitin binding
metal ion binding
ubiquitin protein ligase activity
molecular_function
acid-amino acid ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
ubiquitin-protein ligase
protein modifying enzyme
DNA repair
double-strand break repair
double-strand break repair via nonhomologous end joining
chromatin organization
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to ionizing radiation
protein ubiquitination
negative regulation of transcription elongation from RNA polymerase II promoter
sperm chromatin condensation
interstrand cross-link repair
regulation of gene expression, epigenetic
signal transduction in response to DNA damage
isotype switching
positive regulation of DNA repair
cell division
protein autoubiquitination
protein K63-linked ubiquitination
protein K48-linked ubiquitination
protein K6-linked ubiquitination
DNA repair-dependent chromatin remodeling
positive regulation of double-strand break repair via homologous recombination
protein localization to site of double-strand break
mitotic nuclear division
spermatid development
biological_process
histone H2A ubiquitination
histone H2B ubiquitination
histone exchange
histone H2A K63-linked ubiquitination
cell cycle
chromatin modification
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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