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Invitrogen
ISG15 Recombinant Rabbit Monoclonal Antibody (2C6V1)
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FIGURE: 1 / 2
ISG15 Antibody (MA5-51536) in WB
Western blot analysis of ISG15 in extracts of various cell lines. Samples were incubated with ISG15 Recombinant monoclonal antibody (Product # MA5-51536) using a dilution of 1:1,000 followed by HRP Goat Anti-Rabbit IgG (H+L) secondary antibody at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3 min.
Product Details
MA5-51536
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
2C6V1
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Ubiquitin-like protein ISG15 plays a key role in the innate immune response to viral infection either via its conjugation to a target protein (ISGylation) or via its action as a free or unconjugated protein. It is secreted from monocytes in response to type I interferons and causes natural killer (NK)-cell proliferation and an augmentation of non-MCH (major histocompatibility complex)-restricted cytotoxicity. Synthesis is stimulated by IFN-alpha or IFN-beta or IFN-omega, but not IFN-gamma. ISG15 expression is also induced by overexpression of interferon regulatory factors that participate in transcriptional regulation of IFN genes, and by influenza B virus. ISG15 is secreted by cell lines of monocyte, T-lymphocyte, B-lymphocyte, human fibroblasts, and epithelial origins. Enhancement of NK cell proliferation, augmentation of non-major histocompatibility complex-restricted cytotoxicity, and induction of IFN-gamma from T cells identify ISG15 as a member of the cytokine cascade and suggest that it may be responsible for amplifying and directing some of the immunomodulatory effects of IFN-alpha or IFN-beta. ISG15 has has also been shown to function intracellularly as a ubiquitin homolog. Mutations affecting the gene can result in Immunodeficiency 38, with basal ganglia calcification (IMD38).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 17-kDa protein; hUCRP; interferon, alpha-inducible protein; interferon, alpha-inducible protein (clone IFI-15K); Interferon-induced 15 kDa protein; interferon-induced 15-KDa protein; Interferon-induced 17 kDa protein; interferon-induced 17-kDa/15-kDa protein; interferon-stimulated protein (15 kDa); interferon-stimulated protein 15; interferon-stimulated protein, 15 kDa; IP17; Ubiquitin cross-reactive protein; Ubiquitin-like protein ISG15
Gene Aliases: G1P2; hUCRP; IFI15; IGI15; IMD38; IP17; Irfp; ISG15; UCRP
UniProt ID: (Human) P05161, (Mouse) Q64339
Entrez Gene ID: (Human) 9636, (Rat) 298693, (Mouse) 100038882
immune system process
regulation of immune system process
integrin-mediated signaling pathway
response to virus
response to bacterium
protein ubiquitination
modification-dependent protein catabolic process
positive regulation of bone mineralization
negative regulation of protein ubiquitination
ISG15-protein conjugation
positive regulation of protein oligomerization
regulation of interferon-gamma production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
response to type I interferon
defense response to bacterium
negative regulation of viral genome replication
innate immune response
positive regulation of erythrocyte differentiation
positive regulation of multicellular organismal process
defense response to virus
negative regulation of type I interferon-mediated signaling pathway
protein localization to mitochondrion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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