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PD-1 (CD279) (Nivolumab Biosimilar) Recombinant Rabbit Monoclonal Antibody (5C4.B8 (Nivolumab))
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FIGURE: 1 / 2
PD-1 (CD279) (Nivolumab Biosimilar) Antibody (MA5-51619) in Flow
Flow cytometry analysis of Nivolumab in Cynomolgus monkey lymphocytes using PD-1 (CD279) (Nivolumab Biosimilar) Recombinant Rabbit Monoclocal Antibody (Product # MA5-51619). Cells were stained with an isotype control (Product # MA5-47752, red) or primary antibody (Product # MA5-51619, blue) at a concentration of 1 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated d... View More
Product Details
MA5-51619
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cynomolgus monkey,
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
5C4.B8 (Nivolumab)
Immunogen
Recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1-167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Nivolumab binds to the extracellular portion of human PD-1 (2.6 nM - Scatchard analysis and SPR) - the antibody also binds to cynomolgus PD-1 with a similar affinity (3.9 nM - SPR). The antibody does not bind to other immunoglobulin superfamily proteins such as CD28, CTLA-4, ICOS and BTLA. The epitope of Nivolumab for both human and cynomolgus PD-1 includes the sequences SFVLNWYR-MSPSNQTDKLAAFPEDR (aa 29-53) and SGTYLCGAISLAPKAQIKE (aa 85-103), as shown by mass spectrometry of protease-treated fragments of PD-1 - these residues are thought to additionally be important for liand binding to PD-1. PD-1 is an inhibitory receptor expressed on the surface of T cells. It is able to bind to its ligands PDL-1 and PDL-2 which results in an inhibitory signal leading to decreased T cell proliferation, cytokine protuction and cytotoxic activtiy. PDL-1 is often expressed in human tumors such as melanoma, lung and kidney where it is able to overactivate PD-1 and plays a role in the evasion of cancer cells from the immune system.
Preparation: Recombinant human PD-1-Fc protein consisting of the extracellular domain of PD-1 (amino acids 1-167) and the Fc portion of human IgG1, and Chinese hamster ovary (CHO) cells expressing human PD-1. Nivolumab was generated by grafting the variable regions of PD1.5 onto human kappa and IgG4 constant regions containing an S228P mutation (prevents Fab arm exchange with endogenous IgG4 antibodies).
MA5-51619 is a chimeric antibody. The variable regions from one species have been grafted to the backbone of the other species.
Target Information
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD279; CD279; PD-1; hPD1; PD-1; PD1; alternatively spliced; PDCD1; programmed cell death 1 protein; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279; systemic lupus erythematosus susceptibility 2; unnamed protein product
Gene Aliases: AIMTBS; CD279; hPD-1; hPD-l; hSLE1; PD-1; PD1; PDCD1; SLEB2
UniProt ID: (Human) Q15116
Entrez Gene ID: (Cynomolgus monkey) 102123659, (Human) 5133
B cell apoptotic process
adaptive immune response
immune system process
negative regulation of tolerance induction
negative regulation of T cell mediated immune response to tumor cell
negative regulation of B cell apoptotic process
apoptotic process
humoral immune response
regulation of immune response
negative regulation of immune response
negative regulation of T cell activation
negative regulation of multicellular organismal process
positive regulation of T cell apoptotic process
regulatory T cell apoptotic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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