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Invitrogen
Fibronectin Recombinant Rabbit Monoclonal Antibody (23GB1335)
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FIGURE: 1 / 5
Fibronectin Antibody (MA5-53988) in ICC/IF
Immunocytochemistry analysis of Fibronectin in HT-1080 cells. Nuclei were stained blue with DAPI; Fibronectin recombinant monoclonal antibody (Product # MA5-53988) with a dilution of 1:1,000 was stained magenta with Alexa Fluor 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Product Details
MA5-53988
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
23GB1335
Immunogen
A synthesized peptide derived from human Fibronectin (1-2477AA).
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Fibronectin is a disulfide-bonded dimer with a molecular weight range of 230-250 kDa. In the extracellular matrix of several connective tissues and vessels, fibronectin is present as an insoluble protein that is extensively cross-linked by interchain disulfide bonds forming high molecular mass polymers. Fibronectin is most abundant during embryonic development and tissue remodeling. Fibronectin is also present at high concentrations as a soluble plasma protein. Fibronectin is organized as a linear series of repeating modules which form domains for interaction with fibronectin itself, other matrix components (e.g. collagen and heparin) and receptors on cells (e.g. integrins). Fibronectin is present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Fibronectin has been implicated in carcinoma development in lung cancer. Further, Fibronectin expression is increased especially in non-small cell lung carcinoma. The adhesion of lung carcinoma cells to Fibronectin enhances tumorgenecity and confers resistance to apoptosis induced by standard chemotherapeutic agents. The gene encoding Fibronectin has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some Fibronectin variants has not been determined.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CIG; Cold insoluble globulin (CIG); Cold-insoluble globulin; DKFZp686F10164; DKFZp686H0342; DKFZp686I1370; DKFZp686O13149; epididymis secretory sperm binding protein; extracellular matrix protein; ferritin L subunit; ferritin L-chain; Fibronectin; FN; lnc-ABCA12-8; Migration stimulating factor (MSF); migration-stimulating factor; unnamed protein product
Gene Aliases: CIG; ED-B; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF
UniProt ID: (Human) P02751
Entrez Gene ID: (Human) 2335
angiogenesis
regulation of protein phosphorylation
acute-phase response
cell-substrate junction assembly
cell adhesion
cell-matrix adhesion
calcium-independent cell-matrix adhesion
integrin-mediated signaling pathway
nervous system development
heart development
positive regulation of cell proliferation
regulation of cell shape
response to wounding
positive regulation of gene expression
response to muscle activity
integrin activation
substrate adhesion-dependent cell spreading
endodermal cell differentiation
wound healing
endothelial cell migration
positive regulation of axon extension
positive regulation of fibroblast proliferation
interaction with symbiont
positive regulation of protein kinase B signaling
regulation of ERK1 and ERK2 cascade
negative regulation of transforming growth factor beta production
blood coagulation, fibrin clot formation
negative regulation of monocyte activation
neural crest cell migration involved in autonomic nervous system development
positive regulation of substrate-dependent cell migration, cell attachment to substrate
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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