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Invitrogen
POLB Recombinant Rabbit Monoclonal Antibody (24GB1475)
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FIGURE: 1 / 4
POLB Antibody (MA5-57383) in ICC/IF
Immunocytochemistry analysis of POLB in C2C12 cells. Sample was incubated with POLB monoclonal (Product # MA5-57383) antibody at a dilution of 1:1,000, followed by DAPI and Alexa Fluor 647. Protein abundance based on laser intensity and Smart gain: Low. Scale bar, 20 μm.
Product Details
MA5-57383
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
24GB1475
Immunogen
A synthesized peptide derived from human DNA polymerase beta (aa 300-335)
Conjugate
Form
Liquid
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Target Information
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; MGC125976; POL B; Pol beta; POLB; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; polymerase-beta; unnamed protein product
Gene Aliases: A430088C08Rik; POLB
UniProt ID: (Human) P06746, (Rat) P06766, (Mouse) Q8K409
Entrez Gene ID: (Human) 5423, (Rat) 29240, (Mouse) 18970
DNA binding
damaged DNA binding
DNA-directed DNA polymerase activity
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
transferase activity
nucleotidyltransferase activity
lyase activity
enzyme binding
DNA polymerase activity
metal ion binding
5'-deoxyribose-5-phosphate lyase activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
DNA-directed DNA polymerase
in utero embryonic development
DNA replication
DNA-dependent DNA replication
DNA repair
base-excision repair
base-excision repair, gap-filling
pyrimidine dimer repair
nucleotide-excision repair, DNA gap filling
double-strand break repair via nonhomologous end joining
apoptotic process
inflammatory response
cellular response to DNA damage stimulus
salivary gland morphogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
response to gamma radiation
somatic diversification of immunoglobulins
somatic hypermutation of immunoglobulin genes
response to ethanol
lymph node development
spleen development
homeostasis of number of cells
neuron apoptotic process
response to hyperoxia
immunoglobulin heavy chain V-D-J recombination
DNA biosynthetic process
aging
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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