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Invitrogen
Nephrin Recombinant Rabbit Monoclonal Antibody (1S4U2)
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FIGURE: 1 / 1
Nephrin Antibody (MA5-58070) in WB
Western blot analysis of Nephrin in A-549 cell lysates (25 µg/lane). Samples were incubated overnight at 4°C with Nephrin Recombinant Monoclonal Antibody (Product # MA5-58070) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 90 seconds.
Product Details
MA5-58070
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1S4U2
Immunogen
Recombinant protein (or fragment)
Conjugate
Form
Liquid
Concentration
1.05 mg/mL
Amount
105 µg
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
Product Specific Information
Immunogen sequence: LGDSGLADKG TQLPITTPGL HQPSGEPEDQ LPTEPPSGPS GLPLLPVLFA LGGLLLLSNA SCVGGVLWQR RLRRLAEGIS EKTEAGSEED RVRNEYEESQ WTGERDTQSS TVSTTEAEPY YRSLRDFSPQ LPPTQEEVSY SRGFTGEDED MAFPGHLYDE VERTYPPSGA WGPLYDEVQM GPWDLHWPED TYQDPRGIYD QVAGDLDTLE PDSLPFELRG HLV
Target Information
Primary steroid resistant nephrotic syndrome (SRNS) is characterized by childhood onset of proteinuria and progression to end stage renal disease. Kidney podocytes and their slit diaphragms form the final barrier to urinary protein loss. Congenital nephrotic syndrome (CNS) is caused by mutations in NPHS1 (nephrin) or NPHS2. Nephrin, a recently identified protein is a member of a group of podocyte proteins that constitute major component of the slit diaphragm especially in the foot process. Nephrin, a cell adhesion molecule, may play a crucial role in maintaining the glomerular filtration barrier. Recent studies have suggested that mutations in the gene for Nephrin reportedly lead to congenital nephrosis. Three novel podocyte proteins, Podocin, Nephrin and alpha Actinin 4 have been identified in congenital and experimental models of proteinuria. The role of Nephrin in anti apoptotic activity in podocyte slit diaphragm is believed to be associated with vascular endothelial derived growth factors VEGF signaling. Nephrin seems to play a role in the development or function of the kidney glomerular filtration barrier and it may anchor the podocyte slit diaphragm to the actin cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Nephrin; nephrosis 1, congenital, Finnish type (nephrin); NPHS1; NPHS1, nephrin; Renal glomerulus-specific cell adhesion receptor
Gene Aliases: CNF; nephrin; NPHN; NPHS1
UniProt ID: (Human) O60500
Entrez Gene ID: (Human) 4868
MAPK cascade
cell adhesion
JNK cascade
muscle organ development
skeletal muscle tissue development
myoblast fusion
gene expression
positive regulation of actin filament polymerization
glomerular basement membrane development
protein localization to synapse
slit diaphragm assembly
glomerular visceral epithelial cell development
cell-cell adhesion
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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