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Invitrogen
Thioredoxin 1 Recombinant Rabbit Monoclonal Antibody (3G7)
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FIGURE: 1 / 1
Thioredoxin 1 Antibody (MA5-60658) in WB
Western blot analysis of Thioredoxin 1 in various lysates. Samples were incubated with Thioredoxin 1 Recombinant Monoclonal antibody (Product # MA5-60658) using a dilution of 1:2,000 followed by Goat polyclonal to rabbit IgG at a dilution of 1:50,000. Lane 1: Hela whole cell lysate; Lane 2: MCF-7 whole cell lysate; Lane 3: HepG2 whole cell lysate; Lane 4: A549 whole cell lysate; Lane 5: Rat brain tissue. Predicted band size: 10, 12 kDa; Observed band size: 12 kDa.
Product Details
MA5-60658
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293F
Class
Recombinant Monoclonal
Type
Antibody
Clone
3G7
Immunogen
A synthesized peptide derived from human TRX1.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
25 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Thioredoxins (Trx) are small, multi-functional proteins with oxidoreductase activity and are ubiquitous in essentially all living cells. Trx contains a redox-active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The two cysteine residues in the conserved active centers can be oxidized to form intramolecular disulfide bonds. Reduction of the active site disulfide in oxidized Trx is catalyzed by Trx reductase with NADPH as the electron donor. The reduced Trx is a hydrogen donor for ribonucleotide reductase, the essential enzyme for DNA synthesis, and a potent general protein disulfide reductase with numerous functions in growth and redox regulations. Specific protein disulfide targets for reduction by Trx include protein disulfide-isomerase (PDI) and a number of transcription factors such as p53, NF-kB and AP-1 (T1-151). Trx is also capable of removing H2O2, particularly when it is coupled with either methionine sulfoxide reductase or several isoforms of peroxiredoxins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ADF; ATL-derived factor; DKFZp686B1993; MGC61975; oxidoreductase; RP11-427L11.1; SASP; Surface-associated sulphydryl protein; testicular tissue protein Li 199; Thioredoxin; thioredoxin delta 3; Trx; TRX1; TXN; TXN delta 3; unnamed protein product
Gene Aliases: TRDX; TRX; TRX1; Trx80; TXN; TXN1
UniProt ID: (Human) P10599, (Rat) P11232
Entrez Gene ID: (Human) 7295, (Rat) 116484
negative regulation of transcription from RNA polymerase II promoter
response to radiation
cellular homeostasis
positive regulation of DNA binding
cell redox homeostasis
negative regulation of protein export from nucleus
positive regulation of protein kinase B signaling
cellular detoxification of hydrogen peroxide
response to nitric oxide
transcription, DNA-templated
regulation of transcription, DNA-templated
glycerol ether metabolic process
response to selenium ion
response to activity
positive regulation of peptidyl-serine phosphorylation
regulation of protein import into nucleus, translocation
cellular response to drug
response to drug
response to axon injury
oxidation-reduction process
cellular response to antibiotic
cellular response to glucose stimulus
cellular response to hyperoxia
response to dexamethasone
response to thyroxine
negative regulation of hydrogen peroxide-induced cell death
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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