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RNF168 Recombinant Rabbit Monoclonal Antibody (PSH07-38)
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FIGURE: 1 / 8
RNF168 Antibody (MA562405) in ICC/IF
Knockdown of RNF168 was achieved by transfecting HeLa cells with specific siRNA (Silencer® select Product # s533834, s533835). Immunofluorescence analysis was performed on HeLa cells (untransfected, panels a to d), transfected with RNF168 specific siRNA (panels i to l) or non-specific scrambled siRNA (panels e to h). Cells were fixed, permeabilized, and labeled with RNF168 Recombinant Rabbit Monoclonal Antibody (PSH07-38) (Product # MA562405, 1:500 dilution), followed by Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody... View More
Product Details
MA562405
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
PSH07-38
Immunogen
Recombinant protein within human RNF168 aa 1-571.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: Human breast cancer tissue, human esophagus tissue, mouse brain tissue, rat brain tissue, HeLa.
Tissue Specificity: Low tissue specificity.
Subcellular Location: Nucleus.
Sequence Similarities: 64.6% Mouse/Rat.
Predicted band size: 65 kDa.
Target Information
RNF168 was identified as a chromatin-associated RING finger protein that acts as a ubiquitin ligase both in vitro and in vivo. RNF168 targets histones H2A and H2AX, but not H2B, forming K63 polyubiquitin chains. Upon formation of DNA double strand breaks, RNF168 is recruited to the site of DNA damage where it co-localizes with -gammaH2AX and 53BP1 in an RNF8-dependent manner. This localization of RNF168 increases the local concentration of ubiquinated proteins to the threshold required for retention of the proteins 53BP1 and BRCA1, facilitating the downstream signaling cascade. Thus, RNF168 defines a new pathway demonstrating a functional cooperation between E3 ligases in genome maintenance. At least three isoforms of RNF168 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: E3 ubiquitin-protein ligase RNF168; FLJ35794; hRNF168; RING finger protein 168; ring finger protein 168, E3 ubiquitin protein ligase; ring fnger protein 168; RING-type E3 ubiquitin transferase RNF168; unnamed protein product
Gene Aliases: 3110001H15Rik; hRNF168; RIDL; RNF168
UniProt ID: (Human) Q8IYW5, (Mouse) Q80XJ2, (Rat) B2RYR0
Entrez Gene ID: (Human) 165918, (Mouse) 70238, (Rat) 690043
chromatin binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
transferase activity
nucleosome binding
histone binding
ubiquitin binding
metal ion binding
ubiquitin protein ligase activity
K63-linked polyubiquitin binding
histone ubiquitin ligase activity
histone H2AK15 ubiquitin ligase activity
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
molecular_function
DNA repair
double-strand break repair
ubiquitin-dependent protein catabolic process
cellular response to DNA damage stimulus
response to ionizing radiation
protein ubiquitination
chromatin modification
negative regulation of transcription elongation from RNA polymerase II promoter
histone H2A monoubiquitination
histone H2A-K13 ubiquitination
histone H2A-K15 ubiquitination
isotype switching
positive regulation of DNA repair
protein K63-linked ubiquitination
histone H2A K63-linked ubiquitination
biological_process
DNA damage checkpoint
DNA metabolic process
double-strand break repair via nonhomologous end joining
chromatin organization
response to radiation
interstrand cross-link repair
regulation of gene expression, epigenetic
double-strand break repair via classical nonhomologous end joining
DNA repair-dependent chromatin remodeling
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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