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Invitrogen
Cyclophilin A Polyclonal Antibody
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FIGURE: 1 / 23
Cyclophilin A Antibody (PA1-025) in ICC/IF
Immunofluorescence analysis of Cyclophilin A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cyclophilin A Rabbit Polyclonal Antibody (Product # PA1-025) at 1:250 dilution in 0.1% BSA and incubated for ... View More
Product Details
PA1-025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse
Published species
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Purified recombinant human CyPA.
Conjugate
Form
Liquid
Purification
Ammonium sulfate precipitation
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-025 detects recombinant human cyclophilin A (CyPA), but does not detect endogenous levels of CyPA. PA1-025 also detects the CyPA protein from CHO cells.
PA1-025 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects a prominent ~18 kDa protein representing recombinant human CyPA.
The PA1-025 immunogen is recombinant human CyPA expressed in E. coli.
Target Information
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins. Within the cyclophilin family, there are several different proteins which show a high degree of homology including CyPA, CyPB and CyPC. CyPA, also termed CyP-18, is the most abundant and ubiquitous cyclophilin found in all vertebrate tissues and is present in T-cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclophilin; Cyclophilin A; Cyclosporin A-binding protein; CyP A; EC 5.2.1.8; epididymis secretory sperm binding protein Li 69p; HGNC:9253; MGC117158; MGC12404; MGC23397; Peptidyl-prolyl cis-trans isomerase A; peptidylprolyl isomerase A (cyclophilin A); PPIA; PPIase; PPIase A; rotamase; Rotamase A; SP18; T cell cyclophilin; unnamed protein product
Gene Aliases: 2700098C05; Cphn; CyP-18; CYPA; CYPH; HEL-S-69p; I79_011869; PPIA
UniProt ID: (Human) P62937, (Mouse) P17742, (Chinese hamster) P14851
Entrez Gene ID: (Human) 5478, (Mouse) 268373, (Chinese hamster) 100769490
protein peptidyl-prolyl isomerization
negative regulation of protein phosphorylation
positive regulation of protein phosphorylation
protein folding
negative regulation of protein kinase activity
apoptotic process
viral release from host cell
platelet activation
neuron differentiation
neutrophil chemotaxis
leukocyte chemotaxis
activation of protein kinase B activity
negative regulation of stress-activated MAPK cascade
lipid particle organization
cellular response to oxidative stress
endothelial cell activation
positive regulation of MAPK cascade
regulation of viral genome replication
positive regulation of viral genome replication
positive regulation of protein secretion
positive regulation of NF-kappaB transcription factor activity
cell adhesion molecule production
negative regulation of protein K48-linked ubiquitination
platelet aggregation
negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway
negative regulation of viral life cycle
regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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