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Invitrogen
Cyclophilin B Polyclonal Antibody
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FIGURE: 1 / 38
Cyclophilin B Antibody (PA1-027A) in ICC/IF
Immunofluorescent analysis of Cyclophilin B (green) showing staining in the in the cytoplasm of A431 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cyclophilin B polyclonal antibody (Product # PA1-027A) ... View More
Product Details
PA1-027A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat
Published species
Fruit fly,
Guinea pig,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-027A detects cyclophilin B (CyPB) in mouse, rat, canine, and human tissues.
PA1-027A has been successfully used in Western blot procedures, IF/ICC and IHC (P). By Western blot, this antibody detects a ~19 kDa protein representing CyPB from rat liver extract.
The PA1-027A immunogen is a synthetic peptide corresponding to residues C(194) G K I E V E K P F A I A K E(208) of human CyPB. This sequence is almost completely conserved between human, mouse, rat, bovine, and chicken. PA1-027A immunizing peptide (Cat. # PEP-058) is available for use in neutralization and control experiments.
Target Information
Immunophilins are a family of soluble cytosolic receptors capable of binding to one of two major immunosuppressant agents - cyclosporin A (CsA) or FK506. Proteins which bind FK506 are termed FK506 Binding Proteins (FKBP's) and those which bind cyclosporin A are called cyclophilins (CyP). Both CyP:CsA and FKBP:FK506 complexes have been shown to inhibit calcineurin, a calcium and calmodulin dependent protein phosphatase which has been implicated as an important signaling enzyme in T-cell activation. Thus, providing a possible mechanism of immunosuppression by CsA and FK506. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immunosuppressant compounds. As PPIase's, immunophilins accelerate folding of some proteins both in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline containing proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cyclophilin B; cyclophilin-like protein; Cyp B; CYP-S1; EC 5.2.1.8; epididymis secretory protein Li 39; Peptidyl-prolyl cis-trans isomerase B; peptidylprolyl isomerase B (cyclophilin B); PPIase; PPIase B; PPIB; Rotamase; Rotamase B; S-cyclophilin; S-cyclophylin; SCYLP; unnamed protein product
Gene Aliases: AA408962; AA553318; AI844835; Cphn-2; Cphn2; CyP-20b; CYP-S1; CYPB; HEL-S-39; OI9; PPIB; SCYLP
UniProt ID: (Human) P23284, (Rat) P24368, (Mouse) P24369
Entrez Gene ID: (Human) 5479, (Dog) 478337, (Rat) 64367, (Mouse) 19035
protein folding
neutrophil chemotaxis
positive regulation of multicellular organism growth
positive regulation by host of viral process
positive regulation by host of viral genome replication
protein stabilization
bone development
regulation of post-translational protein modification
protein peptidyl-prolyl isomerization
nuclear transport
chaperone-mediated protein folding
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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