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Invitrogen
SynGAP Polyclonal Antibody
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FIGURE: 1 / 43
SynGAP Antibody (PA1-046) in ICC/IF
Immunofluorescence analysis of SynGAP was performed using 70% confluent log phase PC-12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SynGAP Rabbit Polyclonal Antibody (Product # PA1-046) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product #... View More
Product Details
PA1-046
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
GST fusion protein of a recombinant fragment, residues 947-1167, of rat SynGAP.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-046 detects synaptic Ras-GTPase activating protein (SynGAP) from mouse and rat tissues.
The PA1-046 has been successfully used in Western blot and immunocytochemistry procedures. By Western blot, this antibody detects an ~135 kDa protein representing SynGAP from mouse brain tissue homogenate. Immunocytochemical staining of SynGAP in rat hippocampal neurons with PA1-046 yields a pattern consistent with vesicular staining.
PA1-046 immunogen is residues 947-1167 of rat SynGAP.
Target Information
Synaptic Ras-GTPase activating protein (SynGAP) is a PDZ-domain interacting protein expressed almost exclusively in the brain. SynGAP has been shown to be a major component of postsynaptic density and associates with post synaptic density 95 kDa (PSD-95), a synaptic scaffold protein, and synaptic N-methyl-D-aspartate (NMDA) receptors. SynGAP Ras-GTPase activity has also been shown to be inhibited by phosphorylation by CaM kinase II, another abundant protein located in the postsynaptic density. It has been proposed that SynGAP may function as a negative regulator of MAP Kinase activation in the absence of active NMDA receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp761G1421; Neuronal R; Neuronal RasGAP; OTTHUMP00000209046; p135 SynGAP; Ras GTPase-activating protein SynGAP; Ras/Rap GTPase-activating protein 1; Ras/Rap GTPase-activating protein SynGAP; synaptic Ras GTPase activating protein 1 homolog; synaptic Ras GTPase activating protein, 135kDa; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1
Gene Aliases: Gm1963; KIAA1938; MRD5; RASA1; RASA5; SYNGAP; SYNGAP1
UniProt ID: (Human) Q96PV0, (Mouse) F6SEU4
Entrez Gene ID: (Human) 8831, (Rat) 192117, (Mouse) 240057
Ras protein signal transduction
pattern specification process
axonogenesis
visual learning
dendrite development
receptor clustering
regulation of MAPK cascade
negative regulation of neuron apoptotic process
negative regulation of Ras protein signal transduction
regulation of synaptic plasticity
regulation of long-term neuronal synaptic plasticity
negative regulation of axonogenesis
regulation of synapse structure or activity
modulation of synaptic transmission
neuron apoptotic process
maintenance of postsynaptic specialization structure
regulation of intracellular signal transduction
signal transduction
positive regulation of GTPase activity
regulation of GTPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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