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          • Primary Antibodies ›
          • CDC42 Antibodies

          Invitrogen

          Cdc42 Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          11 References
          View all (19) CDC42 antibodies

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          Cite Cdc42 Polyclonal Antibody

          Additional Information:
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          {{$ctrl.isSkuNotesCollapsed ? 'View more' : 'View less'}}
          • Antibody Testing Data (6)
          • Published Figures (4)
          • Advanced Verification (3)
          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
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          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 13

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          Cdc42 Antibody (PA1-092) in ICC/IF

          Immunofluorescent analysis of cdc42 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a cdc42 polyclonal antibody (Product # PA1-092) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room te... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
          Cdc42 Antibody in Immunocytochemistry (ICC/IF)
          Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Cdc42 Antibody in Western Blot (WB)
          Cdc42 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          Cdc42 Antibody in Western Blot (WB)
          Cdc42 Antibody in Western Blot (WB)
          Cdc42 Antibody in Western Blot (WB)
          Cdc42 Antibody
          Cdc42 Antibody
          Cdc42 Antibody
          Cdc42 Polyclonal Antibody

          Product Details

          PA1-092

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000
          View 6 publications 6 publications

          Immunohistochemistry (IHC)

          -
          View 2 publications 2 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20
          -

          Immunocytochemistry (ICC/IF)

          1:100
          View 2 publications 2 publications

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Non-human primate, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          C-Terminal truncated human recombinant protein
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Protein A

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539858

          Target Information

          Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein; GTP-binding protein G25K; RP1-224A6.5; small GTP binding protein; small GTP binding protein CDC42; unnamed protein product

          View more View less

          Gene Aliases: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS

          View more View less

          UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766

          View more View less

          Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540

          View more View less

          Function(s)
          nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity protein kinase binding GTP-dependent protein binding thioesterase binding GBD domain binding apolipoprotein A-I receptor binding identical protein binding ubiquitin protein ligase activity mitogen-activated protein kinase kinase kinase binding small GTPase
          Process(es)
          heart process cardiac conduction system development cardiac neural crest cell migration involved in outflow tract morphogenesis endocytosis phagocytosis, engulfment actin filament organization Golgi organization regulation of mitotic nuclear division nuclear migration establishment or maintenance of cell polarity signal transduction integrin-mediated signaling pathway small GTPase mediated signal transduction nervous system development protein localization anatomical structure morphogenesis regulation of lamellipodium assembly positive regulation of lamellipodium assembly substantia nigra development establishment of cell polarity actin cytoskeleton organization cell differentiation macrophage differentiation positive regulation of cell growth positive regulation of cell migration positive regulation of pseudopodium assembly negative regulation of protein complex assembly positive regulation of cytokinesis regulation of actin cytoskeleton organization cell junction assembly adherens junction organization embryonic heart tube development establishment or maintenance of apical/basal cell polarity dendritic cell migration neuropilin signaling pathway positive regulation of MAPK cascade modulation by host of viral process establishment of epithelial cell apical/basal polarity filopodium assembly positive regulation of pinocytosis neuron fate determination positive regulation of cellular component organization regulation of filopodium assembly positive regulation of filopodium assembly regulation of stress fiber assembly positive regulation of stress fiber assembly nucleus localization establishment of localization in cell establishment of Golgi localization positive regulation of protein kinase B signaling regulation of attachment of spindle microtubules to kinetochore heart contraction Wnt signaling pathway, planar cell polarity pathway positive regulation of epithelial cell proliferation involved in lung morphogenesis dendritic spine morphogenesis cellular response to interferon-gamma organelle transport along microtubule endothelin receptor signaling pathway involved in heart process regulation of postsynapse organization positive regulation of substrate adhesion-dependent cell spreading positive regulation of protein phosphorylation sprouting angiogenesis keratinocyte development metabolic process response to glucose positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion regulation of exocytosis hair follicle morphogenesis keratinization regulation of protein stability positive regulation of peptidyl-serine phosphorylation cellular protein localization multicellular organism growth regulation of protein catabolic process positive regulation of catalytic activity regulation of protein heterodimerization activity positive regulation of neuron apoptotic process positive regulation of phosphatidylinositol 3-kinase activity positive regulation of DNA replication regulation of protein kinase activity positive regulation of JNK cascade epidermis morphogenesis actin filament bundle assembly regulation of protein metabolic process positive regulation of synapse structural plasticity canonical Wnt signaling pathway submandibular salivary gland formation epithelial-mesenchymal cell signaling hair follicle placode formation positive regulation of hair follicle cell proliferation actin filament branching epithelial cell-cell adhesion positive regulation of intracellular protein transport Rho protein signal transduction endosomal transport
          It has to be done as per old AB suggested Products section.

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