Invitrogen

Cdc42 Polyclonal Antibody

Name: Cdc42 Polyclonal Antibody
Brand: Invitrogen
SKU: PA1-092

Advanced Verification

4 Published Figures

11 References

View all (19) CDC42 antibodies

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Datasheet

Certificates

Protocols

Questions & Answers

Tech Support

Additional Information:

Cdc42 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Cdc42 Antibody (PA1-092) in ICC/IF

Immunofluorescent analysis of cdc42 (green) in HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 5% normal goat serum (Product # 31873) for 15 minutes at room temperature. Cells were probed with a cdc42 polyclonal antibody (Product # PA1-092) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:400 for 30 minutes at room te... View More

Cdc42 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Cdc42 Antibody in Western Blot, Immunohistochemistry (WB, IHC)

Product Image Gallery

Cdc42 Antibody — Fig 2 Knockdown of selected genes required for ANDV infection. Western blots of indicated proteins after knockdown with gene-specific siRNA against (A) dynamin 2 (DNM2); (B) clathrin heavy chain (CLTC); (C) AP2M1; (D) caveolin 1 (CAV1); (E) CDC42; (F) ARF6; (G) NSF; (H) RAB5C; or (I) TSG101; or non-targeting siRNA transfection control (NT). siRNAs were transfected into HMVEC-L at the concentration of 100 nM for 48 h (see Table 1 for more information regarding these genes). The cells were then infected with ANDV (MOI = 0.5) for 24 h or 48 h. Western blots were performed post infection to ensure knockdown of the specific protein expression. Molecular weight of each specific protein is indicated on the right side of each panel. The blots were also probed with beta-actin specific antibody as the gel-loading control. (A) and (B) show only the results collected at 48 h. {KD}

Immunogen image

Immunogen Image Cdc42 Polyclonal Antibody

Product Details

PA1-092

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 6 publications 6 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

Immunocytochemistry (ICC/IF)

1:100

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

C-Terminal truncated human recombinant protein

3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

100 µg

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2539858

Target Information

Rac1 is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for the Rac1 gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: Cell division control protein 42 homolog; cell division cycle 42 (GTP binding protein); cell division cycle 42 homolog; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); G25K GTP-binding protein; growth-regulating protein; GTP binding protein, 25kDa; GTP-binding protein; GTP-binding protein G25K; RP1-224A6.5; small GTP binding protein; small GTP binding protein CDC42; unnamed protein product

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Gene Aliases: AI747189; AU018915; CDC42; CDC42Hs; G25K; TKS

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UniProt ID: (Human) P60953, (Rat) Q8CFN2, (Mouse) P60766

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Entrez Gene ID: (Human) 998, (Rat) 64465, (Mouse) 12540

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Function(s)

nucleotide binding GTPase activity obsolete small monomeric GTPase activity protein binding GTP binding hydrolase activity protein kinase binding GTP-dependent protein binding thioesterase binding GBD domain binding apolipoprotein A-I receptor binding identical protein binding ubiquitin protein ligase activity mitogen-activated protein kinase kinase kinase binding small GTPase

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cdc42 Polyclonal Antibody

Cdc42 Antibody (PA1-092) in ICC/IF

Product Details

PA1-092

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Amount

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Target Information

Bioinformatics

Performance Guarantee

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