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          • Primary Antibodies ›
          • GATA3 Antibodies

          Invitrogen

          GATA3 Polyclonal Antibody

          Advanced Verification
          View all (96) GATA3 antibodies

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          Cite GATA3 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (6)
          • Advanced Verification (1)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          GATA3 Antibody (PA1-101) in ICC/IF

          Immunofluorescent analysis of GATA3 (green) in MCF7 (left panel) and SH-SY5Y cells (right panel). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a GATA3 polyclonal antibody (Product # PA1-101) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunocytochemistry (ICC/IF)
          GATA3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody in Western Blot (WB)
          GATA3 Antibody

          Product Details

          PA1-101

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,500
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:500
          -

          Immunocytochemistry (ICC/IF)

          1-2 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Purified partial recombinant GATA3 protein expressed in E. coli

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          PBS with 1mg/mL BSA, 30% glycerol

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2539874

          Product Specific Information

          Western blot analysis of PA1-101 detects a prominent ~50 kDa protein in GATA3 expressing SH-SY5Y cells. The signal is enhanced in nuclear extracts due to enrichment of GATA3. This antibody has also been validated for Immunofluorescence and IHC (P).PA1-101 was successfully used in western blotting analysis of GATA3 in breast cancer cell lines.

          Target Information

          The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: GATA binding protein-3; GATA-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product

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          Gene Aliases: GATA3; HDR; HDRS

          View more View less

          UniProt ID: (Human) P23771

          View more View less

          Entrez Gene ID: (Human) 2625

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding transcription coactivator binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding transcription factor activity, sequence-specific DNA binding interleukin-2 receptor binding protein binding zinc ion binding identical protein binding sequence-specific DNA binding metal ion binding RNA polymerase II sequence-specific DNA binding transcription factor binding E-box binding HMG box domain binding histone methyltransferase binding sequence-specific double-stranded DNA binding
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis in utero embryonic development cell fate determination neuron migration cell activation type IV hypersensitivity regulation of cytokine production positive regulation of cytokine production kidney development mesonephros development lens development in camera-type eye immune system process immune system development pro-T cell differentiation aortic valve morphogenesis cardiac right ventricle morphogenesis ventricular septum development chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter defense response inflammatory response humoral immune response signal transduction nervous system development axon guidance cell proliferation negative regulation of cell proliferation male gonad development response to xenobiotic stimulus response to virus anatomical structure morphogenesis post-embryonic development positive regulation of signal transduction response to gamma radiation gene expression positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of epithelial to mesenchymal transition regulation of neuron projection development cell differentiation neuron differentiation T cell differentiation erythrocyte differentiation macrophage differentiation regulation of epithelial cell differentiation TOR signaling negative regulation of interferon-gamma production negative regulation of interleukin-2 production positive regulation of interleukin-13 production positive regulation of interleukin-4 production positive regulation of interleukin-5 production T cell differentiation in thymus negative regulation of mammary gland epithelial cell proliferation embryonic hemopoiesis cellular response to interferon-alpha ureter maturation parathyroid hormone secretion norepinephrine biosynthetic process inner ear morphogenesis protein kinase B signaling regulation of neuron apoptotic process ear development response to estrogen thymic T cell selection T-helper 2 cell differentiation innate immune response cell fate commitment response to ethanol positive regulation of T cell differentiation positive regulation of cell differentiation regulation of T-helper cell differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development thymus development embryonic organ development developmental growth anatomical structure formation involved in morphogenesis system development homeostasis of number of cells negative regulation of inflammatory response regulation of developmental process T cell receptor signaling pathway cartilage development positive regulation of protein kinase B signaling parathyroid gland development pharyngeal system development uterus development canonical Wnt signaling pathway mesenchymal to epithelial transition mast cell differentiation ureteric bud formation cellular response to cytokine stimulus cellular response to interleukin-4 cellular response to tumor necrosis factor otic vesicle development cellular response to BMP stimulus positive regulation of ureteric bud formation nephric duct morphogenesis nephric duct formation regulation of nephron tubule epithelial cell differentiation ureter morphogenesis lymphocyte migration cochlea development positive regulation of pri-miRNA transcription from RNA polymerase II promoter regulation of establishment of cell polarity negative regulation of cell motility negative regulation of endothelial cell apoptotic process positive regulation of T-helper 2 cell cytokine production negative regulation of cell proliferation involved in mesonephros development positive regulation of thyroid hormone generation positive regulation of transcription regulatory region DNA binding regulation of cellular response to X-ray negative regulation of fibroblast growth factor receptor signaling pathway involved in ureteric bud formation negative regulation of glial cell-derived neurotrophic factor receptor signaling pathway involved in ureteric bud formation
          It has to be done as per old AB suggested Products section.

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