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Invitrogen
HRV3C Polyclonal Antibody
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FIGURE: 1 / 2
HRV3C Antibody (PA1-118) in WB
Western blot analysis of HRV3c was performed by loading 1 µg of a control protein, either uncleaved (lane 1) or cleaved (lane 2) using the Pierce Human Rhinovirus 3C Protease (HRV 3C) (Product # 88946) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an HRV3c polyclonal antibody (Product # PA1-118) at a dilution of 1:1000 overnight at 4°C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti... View More
Product Details
PA1-118
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Virus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide recognizing the cleavage site of HRV3c protease
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1118 has been used successfully in Western Blot to detect HRV3C cleavage site (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro).
PA1118 has been used in Immunoprecipitation of an HRV3C cleavage site-containing protein from a whole Hela cell lysate.
Target Information
Human rhinovirus 3C protease (HRV3C Protease) is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro. It cleaves between Gln and Gly (independent of Pro). (HRV) 3C Protease is used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is typically dual tagged for easy removal from the sample after cleavage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HRV-3; HRV-3C; HRV3
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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