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Invitrogen
Vimentin Polyclonal Antibody
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FIGURE: 1 / 10
Vimentin Antibody (PA1-16759) in ICC/IF
Immunofluorescence analysis of Vimentin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Vimentin Chicken Polyclonal Antibody (Product # PA1-16759) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Chicken IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Produ... View More
Product Details
PA1-16759
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Chicken,
Horse,
Human,
Mouse,
Pig,
Rat
Published species
Human,
Mouse
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Full length recombinant human Vimentin expressed in and purified from E. coli.
Conjugate
Form
Liquid
Concentration
16.6 mg/mL
Amount
1.66 mg
Purification
IgY fraction
Storage buffer
tissue culture supernatant diluted in PBS
Contains
0.035% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: epididymis secretory sperm binding protein; FLJ36605; Hel113; intermediate filament protein; RP11-124N14.1; unnamed protein product; VIM; VIME; Vimentin; vimentin C-terminus
Gene Aliases: VIM
UniProt ID: (Pig) P02543, (Bovine) P48616, (Human) P08670, (Dog) A0A8C0N8E3, (Mouse) P20152, (Rat) P31000
Entrez Gene ID: (Pig) 100522394, (Bovine) 280955, (Chicken) 420519, (Horse) 100056088, (Human) 7431, (Dog) 477991, (Mouse) 22352, (Rat) 81818
double-stranded RNA binding
structural constituent of cytoskeleton
structural constituent of eye lens
protein domain specific binding
identical protein binding
scaffold protein binding
keratin filament binding
protein binding
RNA binding
binding, bridging
glycoprotein binding
structural molecule activity
protein C-terminus binding
kinase binding
protein kinase binding
protein phosphatase 2A binding
negative regulation of neuron projection development
astrocyte development
positive regulation of collagen biosynthetic process
regulation of mRNA stability
intermediate filament organization
positive regulation of translation
Bergmann glial cell differentiation
SMAD protein signal transduction
lens fiber cell development
cellular response to lipopolysaccharide
cellular response to muramyl dipeptide
cellular response to interferon-gamma
positive regulation of gene expression
intermediate filament-based process
regulation of axonogenesis
positive regulation of glial cell proliferation
regulation of Schwann cell migration
in utero embryonic development
positive regulation of superoxide anion generation
neuron projection development
aging
response to radiation
response to zinc ion
response to selenium ion
response to estradiol
response to vitamin D
skeletal muscle tissue regeneration
cellular response to fibroblast growth factor stimulus
fat cell differentiation
decidualization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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