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Invitrogen
Aurora A Polyclonal Antibody
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FIGURE: 1 / 5
Aurora A Antibody (PA1-26351) in ICC/IF
Immunofluorescence analysis of Aurora A was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Aurora A Polyclonal Antibody (Product # PA1-26351) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight (Panel a: green). Mitotic chromosomes (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Pr... View More
Product Details
PA1-26351
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide representing a portion of the protein encoded within exon 5.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/tris phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-26351 detects Aurora A from human samples.
Target Information
AURKA (Aurora A) is a serine/threonine kinase that regulates mitosis by association in vivo with centrosomes. Overexpression of AURKA is observed in many types of cancer. Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment. Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARK-1; AURAK; Aurora 2; Aurora A Kinase; Aurora kinase A; aurora/IP; aurora/IPL1-like kinase; Aurora/IPL1-related kinase 1; AURORA2; Breast tumor-amplified kinase; EC 2.7.11.1; hARK1; Ipl1- and aurora-related kinase 1; IPL1-related kinase; MGC34538; OTTHUMP00000031341; OTTHUMP00000166072; protein phosphatase 1, regulatory subunit 47; RP5-1167H4.6; serine/threonine protein kinase 15; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A; Serine/threonine-protein kinase Ayk1; unnamed protein product
Gene Aliases: AIK; AIRK1; ARK1; AURA; AURKA; AYK1; BTAK; IAK1; PPP1R47; STK15; STK6; STK7
UniProt ID: (Human) O14965
Entrez Gene ID: (Human) 6790
nucleotide binding
protein kinase activity
protein serine/threonine kinase activity
protein serine/threonine/tyrosine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
protein kinase binding
ubiquitin protein ligase binding
histone kinase activity (H3-S10 specific)
protein heterodimerization activity
protein serine kinase activity
molecular function activator activity
G2/M transition of mitotic cell cycle
meiotic spindle organization
microtubule cytoskeleton organization
mitotic cell cycle
chromatin remodeling
protein phosphorylation
apoptotic process
spindle organization
mitotic spindle organization
spindle assembly involved in female meiosis I
centrosome cycle
mitotic centrosome separation
response to wounding
anterior/posterior axis specification
regulation of G2/M transition of mitotic cell cycle
negative regulation of gene expression
peptidyl-serine phosphorylation
cell projection organization
regulation of protein stability
negative regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
regulation of cytokinesis
regulation of microtubule-based process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
cell cycle G2/M phase transition
positive regulation of mitotic nuclear division
positive regulation of mitotic cell cycle
regulation of centrosome cycle
protein autophosphorylation
cell division
meiotic cell cycle
centrosome localization
cilium disassembly
protein localization to centrosome
positive regulation of cell cycle process
positive regulation of mitochondrial fission
liver regeneration
positive regulation of oocyte maturation
regulation of signal transduction by p53 class mediator
neuron projection extension
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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