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Invitrogen
Nogo-A/B Polyclonal Antibody
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FIGURE: 1 / 6
Nogo-A/B Antibody (PA1-41220) in ICC/IF
Immunofluorescence analysis of Reticulon-4 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Nogo-A/B Polyclonal Antibody (Product # PA1-41220) at (1:100 dilution) in 0.1% BSA, incubated at 4-degree Celsius overnight, and labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Prod... View More
Product Details
PA1-41220
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Sheep,
Rabbit,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50).
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
200 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: human, mouse or rat brain.
Target Information
Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ASY; Foocen; Glut4 vesicle 20 kDa protein; GLUT4 vesicle 20kDa protein; Human NogoA; MGC116054; My043 protein; neurite growth inhibitor 220; Neurite outgrowth inhibitor; Neuroendocrine-specific protein; Neuroendocrine-specific protein C homolog; NI-220/250; Nogo protein; NOGO-A; RTN4-A; NOGO-B; RTN4-B1; NOGO-C; RTN4-C; NSP; reticulon; reticulon 5; Reticulon-4; Reticulon-5; RTN-x; RTN4-Aa; RTN4-Ab; RTN4-B2; RTN4-D; RTN4-E; RTN4-F; RTN4-G; RTN4a; RTN4b; RTN4c; unnamed protein product
Gene Aliases: 1110020G17Rik; AA407876; AA409940; AA960376; ASY; C130026I10Rik; KIAA0886; mKIAA0886; mKIAA4153; My043; Nbla00271; Nbla10545; NgA; NI-250; NI220/250; NOGO; Nogo-A; Nogo-B; Nogo-C; NSP; NSP-CL; r; rat N; rat NogoA; RTN-X; RTN4; RTN4-A; RTN4-B1; RTN4-B2; RTN4-C; SP1507; Vp20
UniProt ID: (Human) Q9NQC3, (Mouse) Q99P72, (Rat) Q9JK11
Entrez Gene ID: (Sheep) 100170399, (Rabbit) 100338467, (Human) 57142, (Mouse) 68585, (Rat) 83765
angiogenesis
nervous system development
axonogenesis
axonal fasciculation
negative regulation of neuron projection development
cerebral cortex radial glia guided migration
negative regulation of cell growth
regulation of cell migration
negative regulation of axon extension
positive regulation of dopamine secretion
negative regulation of neuron differentiation
positive regulation of glial cell differentiation
negative regulation of axonogenesis
regulation of sensory perception of pain
regulation of nervous system development
cardiac epithelial to mesenchymal transition
negative regulation of neuron projection regeneration
endoplasmic reticulum tubular network organization
regulation of branching morphogenesis of a nerve
blastocyst formation
leukocyte migration involved in inflammatory response
apoptotic process
endoplasmic reticulum organization
brain development
positive regulation of epithelial cell migration
central nervous system vasculogenesis
neuron differentiation
positive regulation of mammary gland epithelial cell proliferation
positive regulation of toll-like receptor 9 signaling pathway
positive regulation of Rac protein signal transduction
cell migration involved in vasculogenesis
regulation of apoptotic process
positive regulation of angiogenesis
modulation of synaptic transmission
protein stabilization
nuclear pore complex assembly
positive regulation of protein kinase B signaling
positive regulation of macrophage cytokine production
protein localization to lysosome
cellular response to hypoxia
endoplasmic reticulum tubular network assembly
cellular sphingolipid homeostasis
cell adhesion involved in sprouting angiogenesis
regulation of postsynapse assembly
negative regulation of beta-amyloid formation
positive regulation of neutrophil migration
positive regulation of macrophage migration
positive regulation of protein localization to endoplasmic reticulum
positive regulation of ERBB3 signaling pathway
positive regulation of artery morphogenesis
endoplasmic reticulum tubular network membrane organization
positive regulation of hepatocyte proliferation
negative regulation of vasculogenesis
aging
response to activity
olfactory nerve development
oligodendrocyte differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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