Search
Invitrogen
YAP1 Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 32
YAP1 Antibody (PA1-46189) in ICC/IF
Immunofluorescence analysis of YAP1 was performed using 70% confluent log phase HeLa cells treated with serum starvation for 12 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with YAP1 Polyclonal Antibody (Product # PA1-46189) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Al... View More
Product Details
PA1-46189
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Rat,
Zebrafish
Published species
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Partial recombinant human YAP1 protein expressed in bacteria.
Conjugate
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: brain and kidney lysates, and HEK 293 cell lysate, 293 whole cell lysate, antigen standard for YAP1 (transient overexpression lysate), human kidney protein, human brain protein.
Target Information
YAP (Yes-Associated Protein) is a founding member of the WW domain family of proteins, transcriptional co-activator and oncogene. Its gene is transcriptionally activated in squamous cell carcinoma cells. YAP can act both as a coactivator and corepressor and is the critical downstream regulatory target in the Hippo signaling pathway. The Hippo signaling pathway plays a pivotal role in organ size control and tumour suppression by restricting proliferation and promoting apoptosis. Also plays key roles in tissue tension, 3D tissue shape, cell proliferation, cell death, and cell migration. Mutations in the IRF5 gene results in coloboma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 65 kDa Yes-associated protein; Protein yorkie homolog; transcriptional co-activator; Transcriptional coactivator YAP1; unnamed protein product; yap; Yes associated protein 1; Yes-associated protein 1; Yes-associated protein 1, 65kDa; yes-associated protein 2; Yes-associated protein YAP65 homolog; yes-associated protein, 65 kDa; yorkie homolog
Gene Aliases: AI325207; cb194; CH211-181P1.5-001; COB1; sb:cb194; si:ch211-181p1.5; si:dkey-3b8.3; wu:fc18c04; YAP; YAP-1; YAP1; YAP2; YAP65; YKI; Yorkie; zgc:158380; zYAP
UniProt ID: (Dog) A0A8C0NGY6, (Human) P46937, (Rat) Q2EJA0, (Mouse) P46938, (Zebrafish) Q1L8J7
Entrez Gene ID: (Dog) 479465, (Human) 10413, (Rat) 363014, (Mouse) 22601, (Zebrafish) 561411
transcription regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
chromatin binding
transcription cofactor activity
transcription coactivator activity
transcription corepressor activity
protein binding
proline-rich region binding
DNA-binding transcription factor binding
RNA polymerase II core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
protein C-terminus binding
transcription regulatory region DNA binding
transcription cofactor
negative regulation of transcription from RNA polymerase II promoter
cell morphogenesis
vasculogenesis
blastocyst development
trophectodermal cell differentiation
tissue homeostasis
glandular epithelial cell differentiation
heart process
embryonic heart tube morphogenesis
cellular response to DNA damage stimulus
cell proliferation
positive regulation of cell proliferation
regulation of gene expression
positive regulation of gene expression
negative regulation of gene expression
regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of cell growth
epithelial cell differentiation
negative regulation of epithelial cell differentiation
polarized epithelial cell differentiation
notochord development
response to progesterone
somatic stem cell population maintenance
organ growth
hippo signaling
wound healing
regulation of cell proliferation
negative regulation of fat cell differentiation
positive regulation of osteoblast differentiation
positive regulation of Notch signaling pathway
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of organ growth
paraxial mesoderm development
lateral mesoderm development
epithelial cell proliferation
positive regulation of epithelial cell proliferation
regulation of neurogenesis
positive regulation of cardiac muscle cell proliferation
canonical Wnt signaling pathway
bud elongation involved in lung branching
lung epithelial cell differentiation
intestinal epithelial cell differentiation
intestinal epithelial cell development
regulation of canonical Wnt signaling pathway
cardiac muscle tissue regeneration
canonical glycolysis
protein-containing complex assembly
interleukin-6-mediated signaling pathway
cellular response to retinoic acid
cellular response to gamma radiation
regulation of stem cell proliferation
regulation of metanephric nephron tubule epithelial cell differentiation
positive regulation of canonical Wnt signaling pathway
extrinsic apoptotic signaling pathway
positive regulation of protein localization to nucleus
negative regulation of cilium assembly
positive regulation of stem cell population maintenance
enterocyte differentiation
negative regulation of epithelial cell apoptotic process
negative regulation of stem cell differentiation
negative regulation of extrinsic apoptotic signaling pathway
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
DNA damage induced protein phosphorylation
contact inhibition
negative regulation of nucleic acid-templated transcription
liver development
secondary heart field specification
ventriculo bulbo valve development
retinal pigment epithelium development
brain development
endoderm development
dorsal/ventral axis specification
BMP signaling pathway
pancreas development
fin regeneration
embryonic hemopoiesis
pronephric duct development
glucose homeostasis
negative regulation of apoptotic process
regulation of organ growth
blood vessel morphogenesis
posterior lateral line development
cartilage development
cilium morphogenesis
heart formation
positive regulation of glutamate-ammonia ligase activity
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support