Search
Invitrogen
beta Actin Polyclonal Antibody
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 18
beta Actin Antibody (PA1-46296) in ICC/IF
Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin Rabbit Polyclonal Antibody (Product # PA1-46296) at 2µg/mL in 0.1% BSA and incubated for 3 hours a... View More
Product Details
PA1-46296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Bovine,
Fish,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Bovine,
Human,
Mouse,
Pig,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an N-terminal region of human Beta Actin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is expected to cross-react with a wide range of species based on sequence homology.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Actin is a protein with a high degree of evolutionary conservation that undergoes polymerization to generate filaments, which subsequently assemble into interconnected networks within the cytoplasm of cells.; Actin, cytoplasmic 1; beta actin; beta cytoskeletal actin; Beta-actin; cytoplasmic beta-actin; cytoskeletal beta actin; gamma non-muscle actin; I(2)-actin; PS1TP5-binding protein 1; unnamed protein product
Gene Aliases: ACT-5; ACTB; Actx; Beta-actin; BKRNS; BNS; BRWS1; CSMH; DDS1; E430023M04Rik; PS1TP5BP1; THC8
UniProt ID: (Rabbit) P29751, (Bovine) P60712, (Pig) Q6QAQ1, (Human) P60709, (Rat) P60711, (Mouse) P60710, (Chinese hamster) P48975
Entrez Gene ID: (Rabbit) 100009272, (Bovine) 280979, (Pig) 414396, (Human) 60, (Rat) 81822, (Mouse) 11461, (Chinese hamster) 100689477
protein binding
ATP binding
kinesin binding
Tat protein binding
identical protein binding
nitric-oxide synthase binding
nucleotide binding
structural constituent of cytoskeleton
hydrolase activity
ATPase activity
protein kinase binding
nitric-oxide synthase regulator activity
nucleosomal DNA binding
tau protein binding
structural constituent of postsynaptic actin cytoskeleton
transporter regulator activity
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
morphogenesis of a polarized epithelium
establishment or maintenance of cell polarity
regulation of transmembrane transporter activity
negative regulation of protein binding
adherens junction assembly
apical protein localization
synaptic vesicle endocytosis
regulation of norepinephrine uptake
protein localization to adherens junction
cellular response to cytochalasin B
postsynaptic actin cytoskeleton organization
regulation of protein localization to plasma membrane
chromatin remodeling
regulation of transcription from RNA polymerase II promoter
cytoskeleton organization
axonogenesis
positive regulation of cell proliferation
substantia nigra development
regulation of mitotic metaphase/anaphase transition
maintenance of blood-brain barrier
regulation of apoptotic process
positive regulation of T cell differentiation
negative regulation of cell differentiation
positive regulation of cell differentiation
positive regulation of myoblast differentiation
positive regulation of transcription, DNA-templated
cell motility
positive regulation of norepinephrine uptake
regulation of cell cycle
regulation of G0 to G1 transition
platelet aggregation
regulation of transepithelial transport
regulation of synaptic vesicle endocytosis
positive regulation of stem cell population maintenance
positive regulation of double-strand break repair via homologous recombination
regulation of G1/S transition of mitotic cell cycle
regulation of double-strand break repair
positive regulation of double-strand break repair
regulation of nucleotide-excision repair
retina homeostasis
ATP-dependent chromatin remodeling
retina development in camera-type eye
cellular response to electrical stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support