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          • Primary Antibodies ›
          • LexA Antibodies

          Invitrogen

          LexA Polyclonal Antibody

          1 Reference
          View all (3) LexA antibodies

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          Cite LexA Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (1)
          LexA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          LexA Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          LexA Antibody (PA1-4966) in WB

          Western Blot of LexA in E. coli whole-cell lysate using a LexA Polyclonal Antibody (Product # PA1-4966). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          LexA Antibody in Western Blot (WB)

          Product Details

          PA1-4966

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:3,000
          -

          Immunohistochemistry (IHC)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -

          ChIP assay (ChIP)

          Assay-dependent
          -

          Miscellaneous PubMed (Misc)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Bacteria, Yeast

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Full size LexA recombinant protein.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_667485

          Product Specific Information

          PA1-4966 detects LexA protein in E.coli and yeast samples.

          PA1-4966 has been successfully used in Western blot, immunoprecipitation and immunohistochemical procedures. It is specific for the ~23 kDa LexA protein.

          PA1-4966 immunogen is a highly purified recombinant full size LexA protein.

          Store at -20°C.

          Target Information

          E. coli LexA protein inhibits the transcription of the genes belonging to the SOS regulon that are related to DNA repair and cell division by recognizing and binding to the SOS-box sequence. LexA‘s self-protease activity is promoted by RecA protein which, responding to DNA damage, is activated by its binding to single-strand DNA accumulated in the cells. It is cleaved into two fragments and loses its function as a repressor. As the result, the expression of genes belonging to the SOS regulon is induced, and mutagenic activity in the cells are enhanced.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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