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Invitrogen

CRY1 Polyclonal Antibody

2 Published Figures
3 References
View all (11) CRY1 antibodies

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CRY1 Polyclonal Antibody

Product Details

PA1-527

Applications
Tested Dilution
Publications

Western Blot (WB)

1:2,000
-

Immunohistochemistry (Paraffin) (IHC (P))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

2 µg/mL
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C Q(594) S V G P K V Q R Q S S N(606)
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2276576

Product Specific Information

PA1-527 detects recombinant human cryptochrome 1 (Cry1).

PA1-527 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing full length recombinant human Cry1 protein.

PA1-527 immunizing peptide corresponds to amino acid residues 594-606 from mouse Cry1. PA1-527 immunizing peptide (Cat. # PEP-103) is available for use in neutralization and control procedures.

Target Information

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: cryptochrome 1 (photolyase-like); cryptochrome circadian clock 1; Cryptochrome-1; unnamed protein product

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Gene Aliases: AU020726; AU021000; CRY1; DSPD; PHLL1

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UniProt ID: (Human) Q16526, (Mouse) P97784

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Entrez Gene ID: (Human) 1407, (Mouse) 12952

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Function(s)
nucleotide binding DNA binding double-stranded DNA binding deoxyribodipyrimidine photo-lyase activity DNA (6-4) photolyase activity protein binding photoreceptor activity blue light photoreceptor activity ligand-dependent nuclear receptor binding kinase binding protein kinase binding phosphatase binding histone deacetylase binding E-box binding FAD binding DNA-binding transcription factor binding transcription factor activity, transcription factor binding core promoter sequence-specific DNA binding core promoter binding DNA photolyase activity transcription factor binding nuclear hormone receptor binding ubiquitin binding DNA photolyase
Process(es)
negative regulation of transcription from RNA polymerase II promoter gluconeogenesis regulation of gluconeogenesis circadian rhythm response to light stimulus blue light signaling pathway response to activity lipid storage negative regulation of protein ubiquitination positive regulation of protein ubiquitination response to insulin circadian regulation of gene expression response to glucagon glucose homeostasis regulation of circadian rhythm negative regulation of circadian rhythm signal transduction in response to DNA damage entrainment of circadian clock by photoperiod negative regulation of gluconeogenesis positive regulation of gluconeogenesis negative regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated rhythmic process regulation of DNA damage checkpoint negative regulation of glucocorticoid receptor signaling pathway negative regulation of glucocorticoid secretion DNA repair transcription, DNA-templated regulation of transcription, DNA-templated DNA damage induced protein phosphorylation protein-chromophore linkage response to stimulus
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