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Invitrogen
Ataxin 7 Polyclonal Antibody
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FIGURE: 1 / 24
Ataxin 7 Antibody (PA1-749) in ICC/IF
Immunofluorescence analysis of Ataxin 7 was performed using 70% confluent log phase U-87 MG cells treated with 100 ng of Nocodazole for 16 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Ataxin 7 Rabbit Polyclonal Antibody (Product # PA1-749) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Seco... View More
Product Details
PA1-749
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Fruit fly,
Human
Published species
Fruit fly,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: M(1) S E R A A D D V R G E P R R A A(17) C
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-749 detects ataxin 7 protein from transfected cell lysate.
PA1-749 has successfully been used in immunohistochemistry and Western blot procedures. By Western blot, this antibody detects an ~96 kDa protein representing human ataxin 7 in transfected COS-1 cells.
The PA1-749 immunizing peptide corresponds to amino acid residues 1-17 from human ataxin 7. This sequence is completely conserved in mouse ataxin 7. This peptide (Cat. # PEP-217) is available for use in neutralization and control experiments.
Target Information
Spinocerebellar ataxia type 7 (SCA7) belongs to a class of hereditary neurodegenrative diseases characterized by abnormally expanded polyglutamine (CAG) repeats at the N-terminus. This autosomal dominant cerebellar ataxia primarily affects the cerebellum, retina, and brain stem and causes dementia, macular degeneration and other neurodegenerative characteristics. SCA7 gene encodes the ubiquitous 892 residue protein ataxin-7. This protein is typically located in the cytoplasm and on the nuclear membrane of normal brain neurons. In cells where there is a mutation of the SCA7 gene, ataxin 7 accumulates in intranuclear inclusions and can result in cell death.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ataxin-7; Atxn7-PA; Atxn7-PB; Atxn7-PC; Atxn7-PD; Autosomal dominant cerebellar ataxia with retinal degeneration; CG9866-PA; CG9866-PB; CG9866-PC; CG9866-PD; LD40170p; SAGA associated factor 73 kDa homolog; Spinocerebellar ataxia type 7 protein; unnamed protein product
Gene Aliases: ADCAII; Ataxin7; ATXN7; CG9866; Dmel\CG9866; Dmel_CG9866; OPCA3; SCA7; SGF73
UniProt ID: (Human) O15265, (Fruit fly) Q9VQB6
Entrez Gene ID: (Human) 6314, (Fruit fly) 33423
SAGA complex assembly
positive regulation of histone H2B ubiquitination
microtubule cytoskeleton organization
regulation of DNA repair
regulation of transcription from RNA polymerase II promoter
nucleus organization
negative regulation of microtubule depolymerization
visual perception
regulation of RNA splicing
positive regulation of transcription, DNA-templated
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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