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Invitrogen
RARB Polyclonal Antibody
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FIGURE: 1 / 9
RARB Antibody (PA1-811) in ICC/IF
Immunofluorescence analysis of RARB was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with RARB Polyclonal Antibody (Product # PA1-811) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 (Product # ... View More
Product Details
PA1-811
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse,
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
PBS, whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-811 detects retinoic acid receptor (RAR) beta from human and mouse tissues. This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma.
PA1-811 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~52 kDa protein representing RAR beta from SH-SY5Y (human neuroblastoma) cell extract.
The PA1-811 immunogen is a synthetic peptide corresponding to residues P(429) S V S P S S V E N S G V S Q S P L L Q(448) of mouse RAR beta. This immunizing peptide (Cat. # PEP-005) is available for use in neutralization and control experiments.
Target Information
Retinoic Acid (RA; active metabolite of vitamin A) plays a prominent role in regulating the transition of proliferating precursor cells (such as carcinoma cells and neuronal precursors) to postmitotic differentiated cells. The Retinoid X Receptors (RXRs) family (RXR alpha, beta, and gamma), preferentially bind 9-cis -RA and regulate gene transcription by forming heterodimers with a second family of RA receptors. RAs have been suggested to potentially play a therapeutic role in cervical cancer. RAs are known to play key roles in neuronal development and an increasing body of evidence indicates that retinoid signaling may regulate synaptic plasticity and associated learning and memory behaviors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: HBV-activated protein; hepatitis B virus activated protein; Nuclear receptor subfamily 1 group B member 2; nuclear receptor; RAR beta; open reading frame 1; putative; RAR beta; RAR beta 1'; RAR-b; RAR-beta; RAR-Beta2; RAR-Beta4; RAR-epsilon; RARB; RARbeta isoform 5; retinoic acid; retinoic acid receptor; Retinoic acid receptor beta; retinoic acid receptor, beta polypeptide; unnamed protein product
Gene Aliases: A830025K23; HAP; MCOPS12; NR1B2; RARB; RARbeta; RARbeta1; RRB2
UniProt ID: (Human) P10826, (Mouse) P22605
Entrez Gene ID: (Human) 5915, (Mouse) 218772
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
zinc ion binding
sequence-specific DNA binding
macromolecular complex binding
metal ion binding
retinoid X receptor binding
heterocyclic compound binding
sequence-specific double-stranded DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
steroid hormone receptor activity
retinoic acid receptor activity
drug binding
protein complex binding
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
ureteric bud development
glandular epithelial cell development
outflow tract septum morphogenesis
growth plate cartilage development
regulation of transcription, DNA-templated
apoptotic process
signal transduction
cell proliferation
programmed cell death
striatum development
neurogenesis
cell differentiation
regulation of myelination
negative regulation of chondrocyte differentiation
embryonic hindlimb morphogenesis
multicellular organism growth
positive regulation of apoptotic process
negative regulation of apoptotic process
positive regulation of programmed cell death
positive regulation of transcription from RNA polymerase II promoter
embryonic eye morphogenesis
retinoic acid receptor signaling pathway
embryonic digestive tract development
ventricular cardiac muscle cell differentiation
bone development
negative regulation of cartilage development
neural precursor cell proliferation
stem cell proliferation
negative regulation of stem cell proliferation
retinal pigment epithelium development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
negative regulation of cell proliferation
steroid hormone mediated signaling pathway
positive regulation of neuron differentiation
retina development in camera-type eye
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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