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          • Primary Antibodies ›
          • PPAR gamma-2 Antibodies

          Invitrogen

          PPAR gamma-2 Polyclonal Antibody

          Advanced Verification
          4 Published Figures
          19 References
          View all (2) PPAR gamma-2 antibodies

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          Cite PPAR gamma-2 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          • Published Figures (4)
          • Advanced Verification (1)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
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          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 10

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          PPAR gamma-2 Antibody (PA1-824) in ICC/IF

          Immunofluorescent analysis of PPAR gamma (red) in 3T3-L1 differentiated day 7 cells. The cells were fixed with 4% paraformaldehyde in PBS for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 15 minutes, and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with a PPAR gamma rabbit polyclonal antibody (Product # PA1-824) at a dilution of 1:200 in blocking buff... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Immunocytochemistry (ICC/IF)
          PPAR gamma-2 Antibody in Western Blot (WB)
          PPAR gamma-2 Antibody in Western Blot (WB)
          PPAR gamma-2 Antibody in Western Blot (WB)
          PPAR gamma-2 Antibody
          PPAR gamma-2 Polyclonal Antibody

          Product Details

          PA1-824

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500
          View 10 publications 10 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunocytochemistry (ICC/IF)

          1:500
          View 4 publications 4 publications

          Immunoprecipitation (IP)

          -
          View 1 publication 1 publication

          Gel Shift (EMSA)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse

          Published species

          Human, Mouse, Non-human primate

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2166200

          Product Specific Information

          PA1-824 detects peroxisome proliferator activated receptor (PPAR) gamma 2 from human and mouse tissues. This antibody does not detect PPAR alpha or PPAR delta.

          PA1-824 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~56 kDa protein representing PPAR gamma 2 from NIH-3T3 cell lysate. PA1-824 inhibits PPAR gamma 2 DNA binding.

          PA1-824 immunogen is a synthetic peptide corresponding to residues M(1) G E T L G D S P I D P E S D S(16) C of human PPAR gamma-2. This sequence is not conserved in PPAR gamma-1.

          PA1-824 can be used with blocking peptide PEP-027.

          Target Information

          Peroxisome proliferators are non-genotoxic carcinogens which are purported to exert their effect on cells through their interaction with members of the nuclear hormone receptor family, termed peroxisome proliferator activated receptors (PPARs). Nuclear hormone receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate ligand. Studies indicate that PPARs are activated by peroxisome proliferators such as clofibric acid, nafenopin, and WY-14,643, as well as by some fatty acids. It has also been shown that PPARs can induce transcription of acyl coenzyme A oxidase and cytochrome P450 A6 (CYP450 A6) through interaction with specific response elements. PPAR alpha is activated by free fatty acids including linoleic, arachidonic, and oleic acids. Induction of peroxisomes by this mechanism leads to a reduction in blood triglyceride levels. PPAR alpha is expressed mainly in skeletal muscle, heart, liver, and kidney and is thought to regulate many genes involved in the beta-oxidation of fatty acids. Activation of rat liver PPAR alpha has been shown to suppress hepatocyte apoptosis. PPAR gamma 2, like several other nuclear hormone receptors, heterodimerizes with retinoic X receptor (RXR) alpha to form a transcriptionally competent complex.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Nuclear receptor subfamily 1 group C member 3; peroxisome proliferator; peroxisome proliferator activated receptor; peroxisome proliferator activated receptor gamma 2; peroxisome proliferator activated receptor gamma 4; Peroxisome proliferator-activated receptor gamma; peroxisome proliferator-activated receptor-gamma 5; peroxisome proliferator-activated receptor-gamma splicing; PPAR-gamma; unnamed protein product

          View more View less

          Gene Aliases: CIMT1; FPLD3; GLM1; NR1C3; PPAR-gamma; PPAR-gamma2; PPARG; PPARG1; PPARG2; PPARG5; PPARgamma; PPARgamma2

          View more View less

          UniProt ID: (Human) P37231, (Mouse) P37238

          View more View less

          Entrez Gene ID: (Human) 5468, (Mouse) 19016

          View more View less

          Function(s)
          transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription cofactor binding transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding nucleic acid binding DNA binding chromatin binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding prostaglandin receptor activity protein binding zinc ion binding enzyme binding peptide binding identical protein binding sequence-specific DNA binding metal ion binding retinoid X receptor binding arachidonic acid binding DBD domain binding LBD domain binding WW domain binding alpha-actinin binding R-SMAD binding E-box binding STAT family protein binding DNA-binding transcription factor binding RNA polymerase II regulatory region DNA binding core promoter sequence-specific DNA binding steroid hormone receptor activity transcription factor binding drug binding protein phosphatase binding estrogen receptor binding ligand-dependent nuclear receptor transcription coactivator activity activating transcription factor binding transcription regulatory region DNA binding C4 zinc finger nuclear receptor DNA-binding transcription factor zinc finger transcription factor gene-specific transcriptional regulator
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter placenta development regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter fatty acid metabolic process G-protein coupled receptor signaling pathway response to nutrient regulation of blood pressure hormone-mediated signaling pathway positive regulation of gene expression negative regulation of gene expression negative regulation of macrophage derived foam cell differentiation positive regulation of cholesterol efflux negative regulation of cholesterol storage negative regulation of lipid storage long-chain fatty acid transport negative regulation of angiogenesis cell differentiation monocyte differentiation BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway negative regulation of BMP signaling pathway intracellular receptor signaling pathway epithelial cell differentiation negative regulation of defense response response to nutrient levels positive regulation of cholesterol transport cellular response to insulin stimulus response to lipid peroxisome proliferator activated receptor signaling pathway glucose homeostasis regulation of circadian rhythm mRNA transcription from RNA polymerase II promoter negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration innate immune response cell fate commitment positive regulation of fat cell differentiation negative regulation of osteoblast differentiation positive regulation of lipid metabolic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of fatty acid metabolic process positive regulation of transcription from RNA polymerase II promoter retinoic acid receptor signaling pathway cell maturation rhythmic process negative regulation of smooth muscle cell proliferation negative regulation of inflammatory response white fat cell differentiation regulation of transport negative regulation of multicellular organismal process lipid homeostasis negative regulation of interferon-gamma-mediated signaling pathway negative regulation of SMAD protein import into nucleus positive regulation of adiponectin secretion cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia negative regulation of mitochondrial fission positive regulation of lipoprotein transport nuclear receptor-mediated signaling pathway regulation of cellular response to insulin stimulus negative regulation of extracellular matrix assembly negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of cardiac muscle hypertrophy in response to stress negative regulation of cellular response to transforming growth factor beta stimulus positive regulation of adipose tissue development negative regulation of connective tissue replacement involved in inflammatory response wound healing negative regulation of vascular smooth muscle cell proliferation negative regulation of receptor signaling pathway via STAT positive regulation of vascular associated smooth muscle cell apoptotic process negative regulation of vascular endothelial cell proliferation positive regulation of apoptotic signaling pathway negative regulation of cytokine production response to dietary excess diet induced thermogenesis negative regulation of acute inflammatory response transcription, DNA-templated transcription from RNA polymerase II promoter activation of cysteine-type endopeptidase activity involved in apoptotic process inflammatory response signal transduction negative regulation of cell proliferation response to light stimulus regulation of gene expression negative regulation of receptor biosynthetic process negative regulation of sequestering of triglyceride regulation of lipid metabolic process fatty acid oxidation negative regulation of cell growth response to food response to retinoic acid negative regulation of collagen biosynthetic process lipoprotein transport positive regulation of apoptotic process steroid hormone mediated signaling pathway fat cell differentiation regulation of fat cell differentiation low-density lipoprotein particle receptor biosynthetic process positive regulation of fatty acid oxidation positive regulation of oligodendrocyte differentiation brown fat cell differentiation positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of telomerase activity response to low-density lipoprotein particle positive regulation of phagocytosis, engulfment regulation of transcription involved in cell fate commitment cellular response to lithium ion cellular response to organic cyclic compound negative regulation of peptide hormone secretion negative regulation of cellular response to insulin stimulus negative regulation of pancreatic stellate cell proliferation
          It has to be done as per old AB suggested Products section.

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