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Invitrogen
JunB Polyclonal Antibody
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FIGURE: 1 / 3
JunB Antibody (PA1-835) in ICC/IF
Immunofluorescence analysis of JunB was done on 70% confluent log phase PC-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with JunB Rabbit Polyclonal Antibody (Product # PA1-835) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a... View More
Product Details
PA1-835
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues D(45) P Y R G L K G P G A R G P G P E(61) of mouse JunB.
Conjugate
Form
Lyophilized
Concentration
1 mg/mL
Amount
200 µg
Purification
IgG fraction
Storage buffer
PBS
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-835 detects JunB from mouse cells.
PA1-835 has been successfully used in ChiP, Western blot and immunoprecipitation procedures. By Western blot, this antibody detects an ~38 kDa protein representing JunB from both uninduced and induced L929 cells.
The PA1-835 immunogen is a synthetic peptide corresponding to residues D(45) P Y R G L K G P G A R G P G P E(61) of mouse JunB.
Reconstitute in 200 µL PBS to create a stock of 1 mg/mL.
Target Information
JunB, a member of the Jun cellular oncogene family, is a basic region, leucine zipper transcription factor. It is involved in regulating gene activity following the primary growth factor response. JunB binds to the DNA sequence 5'-TGA[CG]TCA-3'. Cellular oncogenes play critical roles in cellular communication pathways that regulate growth, development, and differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: activator protein 1; Jun B; jun B proto-oncogene; Jun-B oncogene; MyD21; Oncogene Jun-B; Transcription factor AP-1 subunit JunB; transcription factor jun-B; Transcription factor JunB; unnamed protein product
Gene Aliases: AP-1; Jun-b; JUNB
UniProt ID: (Human) P17275, (Mouse) P09450
Entrez Gene ID: (Human) 3726, (Mouse) 16477
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
double-stranded DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
sequence-specific double-stranded DNA binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcription coactivator activity
transcription factor binding
sequence-specific DNA binding
basic leucine zipper transcription factor
vasculogenesis
osteoblast differentiation
in utero embryonic development
trophectodermal cell differentiation
osteoclast proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
cell differentiation
osteoclast differentiation
osteoblast proliferation
regulation of cell proliferation
positive regulation of cell differentiation
positive regulation of transcription from RNA polymerase II promoter
decidualization
response to steroid hormone
regulation of cell cycle
embryonic process involved in female pregnancy
labyrinthine layer blood vessel development
cellular response to calcium ion
integrated stress response signaling
regulation of T-helper 17 cell differentiation
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
transcription from RNA polymerase II promoter
response to radiation
response to mechanical stimulus
cellular process
regulation of cell death
response to lipopolysaccharide
cellular response to hormone stimulus
response to cytokine
response to drug
response to cAMP
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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