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Invitrogen

p300 Polyclonal Antibody

Advanced Verification
2 Published Figures
9 References
View all (22) p300 antibodies

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p300 Polyclonal Antibody

Product Details

PA1-848

Applications
Tested Dilution
Publications

Western Blot (WB)

1-2 µg/mL
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

5 µg/mL
-

Flow Cytometry (Flow)

-
View 1 publication 1 publication

ChIP assay (ChIP)

2.5 µg/10^6 cells
View 4 publications 4 publications

CUT&RUN (CUT&RUN)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325902

Product Specific Information

PA1-848 detects p300 from human cells.

PA1-848 has been successfully used in Western blot and ChIP procedures. By Western blot, this antibody detects an ~265 kDa protein representing p300 from HeLa cell lysate.

The PA1-848 immunogen is a synthetic peptide corresponding to residues G(139) T S G P N Q G P T Q S T(151) C in the nuclear factor binding domain of human p300. The PA1-848 immunizing peptide (Cat. # PEP-053) is available for use in neutralization and control experiments.

Target Information

EP300 encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. p300 is related by sequence to CPB (CREB-binding protein [CREB: cyclic-AMP responsive element binding protein]), and like CPB can stimulate transcription through activation of CREB. This EP300 activity is specifically inhibited by the adenovirus oncoprotein E1A. EP300 has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: E1A binding protein p300; E1A-associated protein p300; E1A-binding protein, 300kD; Histone acetyltransferase p300; Histone butyryltransferase p300; Histone crotonyltransferase p300; p300 HAT; Protein 2-hydroxyisobutyryltransferase p300; Protein isonicotinyltransferase p300; Protein lactyltransferas p300; Protein propionyltransferase p300

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Gene Aliases: EP300; KAT3B; MKHK2; P300; RSTS2

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UniProt ID: (Human) Q09472

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Entrez Gene ID: (Human) 2033

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Function(s)
transcription cofactor binding transcription coactivator binding p53 binding DNA binding chromatin binding damaged DNA binding transcription cofactor activity transcription coactivator activity histone acetyltransferase activity lysine N-acetyltransferase activity, acting on acetyl phosphate as donor protein binding beta-catenin binding zinc ion binding H3 histone acetyltransferase activity H4 histone acetyltransferase activity acetyltransferase activity transferase activity transferase activity, transferring acyl groups ligand-dependent nuclear receptor binding chromatin DNA binding histone acetyltransferase activity (H3-K18 specific) histone H2B acetyltransferase activity histone acetyltransferase activity (H3-K27 specific) metal ion binding tau protein binding androgen receptor binding NF-kappaB binding RNA polymerase II sequence-specific DNA binding transcription factor binding peptide-lysine-N-acetyltransferase activity protein propionyltransferase activity pre-mRNA intronic binding STAT family protein binding peptide 2-hydroxyisobutyryltransferase activity peptide lactyltransferase (CoA-dependent) activity histone lactyltransferase (CoA-dependent) activity acetylation-dependent protein binding peptide crotonyltransferase activity peptide butyryltransferase activity histone crotonyltransferase activity histone butyryltransferase activity DNA-binding transcription factor binding histone reader activity histone H3K122 acetyltransferase activity
Process(es)
autophagosome assembly negative regulation of transcription from RNA polymerase II promoter response to hypoxia somitogenesis thigmotaxis behavioral defense response stimulatory C-type lectin receptor signaling pathway gluconeogenesis glycolytic process regulation of glycolytic process chromatin remodeling regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter protein acetylation internal protein amino acid acetylation apoptotic process I-kappaB kinase/NF-kappaB signaling JAK-STAT cascade nervous system development heart development skeletal muscle tissue development learning or memory circadian rhythm lipid biosynthetic process organ morphogenesis regulation of autophagy negative regulation of autophagy positive regulation of gene expression regulation of mitochondrion organization positive regulation of neuron projection development N-terminal peptidyl-lysine acetylation internal peptidyl-lysine acetylation B cell differentiation platelet formation lung development positive regulation of transforming growth factor beta receptor signaling pathway negative regulation of protein complex assembly protein destabilization cellular response to nutrient levels negative regulation of protein oligomerization cellular response to UV multicellular organism growth megakaryocyte development endodermal cell differentiation swimming TORC1 signaling TORC2 signaling positive regulation of protein import into nucleus intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator protein kinase B signaling response to estrogen positive regulation by host of viral transcription fat cell differentiation negative regulation of gluconeogenesis positive regulation of gene expression, epigenetic positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of JAK-STAT cascade rhythmic process protein stabilization positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of protein kinase B signaling canonical Wnt signaling pathway face morphogenesis regulation of androgen receptor signaling pathway peptidyl-lysine propionylation protein localization to chromatin cellular response to leucine tricarboxylic acid metabolic process T-helper 17 cell lineage commitment regulation of tubulin deacetylation peptidyl-lysine crotonylation peptidyl-lysine butyrylation regulation of cellular response to heat positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of T-helper 17 cell lineage commitment
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