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Invitrogen
DNMT3A Polyclonal Antibody
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Product Details
PA1-882
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Fruit fly,
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues D(86) L L P N G D L E K R S E P Q(100) C of human Dnmt3a.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-882 detects DNA methyltransferase 3a (Dnmt3a) from human and mouse tissues and cells as well as recombinant human Dnmt3a. This antibody does not detect full length recombinant human Dnmt3b or Dnmt1.
PA1-882 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~120 kDa protein representing Dnmt3a from P19 cell nuclear extracts.
The PA1-882 immunogen is a synthetic peptide corresponding to residues D(86) L L P N G D L E K R S E P Q(100) C of human Dnmt3a. This immunizing peptide (Cat. # PEP-116) is available for use in neutralization and control experiments.
Target Information
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AU015806; Cysteine methyltransferase DNMT3A; DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5-)-methyltransferase 3 alpha; DNA cytosine methyltransferase 3A2; DNA methyltransferase HsaIIIA; DNA methyltransferase MmuIIIA; DNA MTase HsaIIIA; DNA MTase MmuIIIA; Dnmt3a; W91664
Gene Aliases: DNMT3A; DNMT3A2; HESJAS; M.HsaIIIA; MmuIIIA; TBRS
UniProt ID: (Human) Q9Y6K1, (Mouse) O88508
Entrez Gene ID: (Human) 1788, (Mouse) 13435
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
chromatin binding
transcription corepressor activity
DNA (cytosine-5-)-methyltransferase activity
protein binding
methyltransferase activity
zinc ion binding
transferase activity
identical protein binding
unmethylated CpG binding
metal ion binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
lncRNA binding
protein-cysteine methyltransferase activity
DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates
DNA methyltransferase
negative regulation of transcription from RNA polymerase II promoter
chromatin organization
methylation-dependent chromatin silencing
spermatogenesis
response to xenobiotic stimulus
response to toxic substance
post-embryonic development
response to ionizing radiation
response to lead ion
regulation of gene expression
neuron differentiation
chromatin silencing by small RNA
heterochromatin assembly
response to nutrient levels
methylation
response to estradiol
response to vitamin A
response to cocaine
DNA methylation involved in embryo development
hypermethylation of CpG island
response to ethanol
negative regulation of transcription, DNA-templated
cellular response to amino acid stimulus
cellular response to ethanol
cellular response to hypoxia
genetic imprinting
hepatocyte apoptotic process
autosome genomic imprinting
transposable element silencing by piRNA-mediated DNA methylation
positive regulation of cellular response to hypoxia
cellular response to bisphenol A
DNA methylation
regulation of gene expression by genetic imprinting
transcription, DNA-templated
regulation of transcription, DNA-templated
maintenance of DNA methylation
DNA methylation on cytosine within a CG sequence
positive regulation of cell death
chromatin modification
DNA methylation on cytosine
DNA methylation involved in gamete generation
S-adenosylhomocysteine metabolic process
S-adenosylmethioninamine metabolic process
C-5 methylation of cytosine
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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