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Invitrogen
Calreticulin Polyclonal Antibody
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FIGURE: 1 / 10
Calreticulin Antibody (PA1-903) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of MCF7 (Lane 1), MDA-MB-231 (Lane 2), HeLa (Lane 3), K-562 (Lane 4), Jurkat (Lane 5) and A549 (Lane 6). The blot was probed with Chicken Anti-Calreticulin Polyclonal Antibody (Product # PA1-903, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Chicken IgY (H+L) Secondary Antibody, HRP conjugate (Product # A16054, 0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to Calreticulin was observed across the cell lines tested. Known quantity... View More
Product Details
PA1-903
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Hamster,
Human,
Mouse,
Rabbit,
Rat
Published species
Amphibian,
Dog,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Chicken
/ IgY
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.
PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.
PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.
Target Information
Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Autoantigen RO; CABP3; CALBP; Calcium-binding protein 3; CALR; Calregulin; Calreticulin; calreticulin precursor; CRP55; Endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; FLJ26680; grp60; HACBP; Ro ribonucleoprotein autoantigen (Ro/SS-A) precursor; Ro/SS-A Antigen; Sicca syndrome antigen A (autoantigen Ro; calreticulin); unnamed protein product
Gene Aliases: CALR; CALR1; Calregulin; cC1qR; CRP55; CRT; CRTC; ERp60; HACBP; HEL-S-99n; I79_008346; RO; SSA
UniProt ID: (Human) P27797, (Rabbit) P15253, (Chinese hamster) Q8K3H7, (Mouse) P14211, (Rat) P18418
Entrez Gene ID: (Rabbit) 100350417, (Human) 811, (Rabbit) 100009050, (Dog) 476694, (Chinese hamster) 100689096, (Mouse) 12317, (Rat) 64202
complement component C1q binding
DNA binding
RNA binding
mRNA binding
nuclear export signal receptor activity
integrin binding
iron ion binding
calcium ion binding
protein binding
zinc ion binding
carbohydrate binding
ubiquitin protein ligase binding
peptide binding
hormone binding
protein binding involved in protein folding
metal ion binding
androgen receptor binding
unfolded protein binding
chaperone binding
molecular sequestering activity
glycoprotein binding
poly(A) RNA binding
chaperone
negative regulation of transcription from RNA polymerase II promoter
peptide antigen assembly with MHC class I protein complex
regulation of transcription, DNA-templated
protein folding
protein export from nucleus
cellular calcium ion homeostasis
spermatogenesis
positive regulation of cell proliferation
response to xenobiotic stimulus
positive regulation of endothelial cell migration
positive regulation of gene expression
negative regulation of translation
cortical actin cytoskeleton organization
response to estradiol
negative regulation of intracellular steroid hormone receptor signaling pathway
response to testosterone
protein localization to nucleus
protein folding in endoplasmic reticulum
ERAD pathway
regulation of meiotic nuclear division
glucocorticoid receptor signaling pathway
regulation of apoptotic process
negative regulation of neuron differentiation
positive regulation of cell cycle
negative regulation of transcription, DNA-templated
negative regulation of retinoic acid receptor signaling pathway
positive regulation of phagocytosis
protein stabilization
sequestering of calcium ion
protein maturation
cardiac muscle cell differentiation
cellular response to electrical stimulus
cellular response to lithium ion
cellular senescence
cellular response to virus
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of trophoblast cell migration
positive regulation of NIK/NF-kappaB signaling
response to peptide
response to glycoside
response to biphenyl
positive regulation of dendritic cell chemotaxis
cell cycle arrest
positive regulation of DNA replication
chaperone-mediated protein folding
response to organic substance
response to drug
cellular response to organic substance
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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