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Invitrogen
IRAK4 Polyclonal Antibody
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FIGURE: 1 / 7
IRAK4 Antibody (PA1-9057) in ICC/IF
Immunocytochemistry analysis of IRAK4 using IRAK4 Polyclonal Antibody (Product # PA1-9057) in paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and microtubule staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).
Product Details
PA1-9057
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to the N terminus amino acids NKPITPSTYVRC (aa 2-14).
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
IL-1 receptor associated kinases (IRAKs) are serine/threonine kinases involved in signaling via Toll/IL-1 pathways. Overexpression of IRAK-4 activates NF-kappa-B and MAPK pathways. IRAK-4 interacts with and phosphorylates IRAK-1. IRAK-4-deficient animals are completely resistant to the challenge with LPS. Animals and humans lacking IRAK-4 are impaired in their responses to viral and bacterial challenges. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses and in innate immunity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: interleukin-1; Interleukin-1 receptor-associated kinase 4; IRAK-4; IRAK-4 mutated form 1; IRAK-4 mutated form 2; IRAK4; protein kinase; IRAK4S; protein kinase; protein kinase; Renal carcinoma antigen NY-REN-64; unnamed protein product
Gene Aliases: IMD67; IPD1; IRAK-4; IRAK4; NY-REN-64; REN64
UniProt ID: (Human) Q9NWZ3
Entrez Gene ID: (Human) 51135
toll-like receptor signaling pathway
immune system process
neutrophil mediated immunity
MyD88-dependent toll-like receptor signaling pathway
signal transduction
JNK cascade
Toll signaling pathway
cytokine-mediated signaling pathway
positive regulation of defense response
lipopolysaccharide-mediated signaling pathway
toll-like receptor 4 signaling pathway
toll-like receptor 9 signaling pathway
intracellular signal transduction
NIK/NF-kappaB signaling
interleukin-33-mediated signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of smooth muscle cell proliferation
interleukin-1-mediated signaling pathway
neutrophil migration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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