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Invitrogen

HSP90 alpha Polyclonal Antibody

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32 Published Figures
53 References
View all (33) HSP90 alpha antibodies

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HSP90 alpha Polyclonal Antibody

Product Details

PA3-013

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 37 publications 37 publications

Immunohistochemistry (IHC)

-
View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL
-

Immunocytochemistry (ICC/IF)

1:50-1:200
View 6 publications 6 publications

Immunoprecipitation (IP)

2 µg
View 4 publications 4 publications

ChIP assay (ChIP)

-
View 1 publication 1 publication
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Sheep, Rat

Published species

Human, Mouse, Non-human primate, Rabbit

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2120934

Product Specific Information

PA3-013 detects heat shock protein 90 (HSP90) from human, monkey, mouse, sheep (adrenal) and rat tissues and cells. This antibody does not detect HSP84.

PA3-013 has been successfully used in Western blot, immunofluorescence, immunohistochemistry (paraffin) and immunoprecipitation procedures. By Western blot, this antibody detects an ~86 kDa protein representing HSP86 from HeLa cell lysate. Immunofluorescence staining of HSP86 in HeLa cells with PA3-013 yields a pattern consistent with cytoplasmic staining. Immunoprecipitation experiments with this antibody suggest that HSP86 exists primarily as homodimers in HeLa cells. Furthermore, the antibody is capable of precipitating HSP86 that is complexed with other proteins such as the aryl hydrocarbon (Ah) receptor.

The PA3-013 immunogen is a synthetic peptide corresponding to residues P(2) E E T Q T Q D Q P M(12) of mouse HSP86. The N-terminal regions of HSP84 and HSP86 show the largest difference in amino acid sequence.

Target Information

Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: epididymis luminal secretory protein 52; epididymis secretory sperm binding protein Li 65p; Heat shock 86 kDa; heat shock 90kD protein 1, alpha; heat shock 90kD protein 1, alpha-like 4; heat shock 90kD protein, alpha-like 4; heat shock 90kDa protein 1, alpha; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; heat shock protein 90kDa alpha family class A member 1; heat shock protein alpha; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; LPS-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen; unnamed protein product

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Gene Aliases: 86kDa; 89kDa; AL024080; AL024147; EL52; HEL-S-65p; Hsp103; hsp4; HSP86; Hsp86-1; Hsp89; HSP89A; Hsp90; HSP90A; HSP90AA1; HSP90N; HSPC1; HSPCA; HSPCAL1; HSPCAL4; HSPN; LAP-2; LAP2

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UniProt ID: (Human) P07900, (Mouse) P07901, (Rat) P82995

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Entrez Gene ID: (Sheep) 100127209, (Human) 3320, (Mouse) 15519, (Rat) 299331

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Function(s)
nucleotide binding UTP binding CTP binding RNA binding mRNA binding protein binding ATP binding GTP binding hydrolase activity ATPase activity sulfonylurea receptor binding protein phosphatase binding MHC class II protein complex binding nitric-oxide synthase regulator activity TPR domain binding ubiquitin protein ligase binding dATP binding identical protein binding protein homodimerization activity histone deacetylase binding protein binding involved in protein folding ion channel binding tau protein binding GTPase binding Rho GDP-dissociation inhibitor binding unfolded protein binding DNA polymerase binding scaffold protein binding disordered domain specific binding ATP-dependent protein folding chaperone enzyme-substrate adaptor activity protein tyrosine kinase binding protein kinase binding poly(A) RNA binding Hsp90 family chaperone
Process(es)
neuron migration activation of innate immune response positive regulation of defense response to virus by host skeletal muscle contraction protein folding mitochondrial transport response to unfolded protein telomere maintenance via telomerase response to heat response to cold response to xenobiotic stimulus response to salt stress positive regulation of lamellipodium assembly cardiac muscle cell apoptotic process protein import into mitochondrial matrix regulation of protein ubiquitination positive regulation of telomere maintenance via telomerase positive regulation of protein polymerization positive regulation of interferon-beta production regulation of protein localization cellular response to heat protein refolding response to cocaine positive regulation of protein import into nucleus regulation of apoptotic process regulation of protein complex assembly protein unfolding response to estrogen positive regulation of nitric oxide biosynthetic process positive regulation of protein catabolic process positive regulation of cell size nitric oxide metabolic process response to antibiotic protein stabilization chaperone-mediated protein complex assembly positive regulation of cardiac muscle contraction chaperone-mediated autophagy cellular response to virus regulation of postsynaptic membrane neurotransmitter receptor levels neurofibrillary tangle assembly telomerase holoenzyme complex assembly nitric oxide biosynthetic process response to stress positive regulation of protein import into nucleus, translocation protein import into mitochondrial outer membrane positive regulation of cytotoxic T cell differentiation regulation of catalytic activity
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