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Invitrogen
SEC61B Polyclonal Antibody
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FIGURE: 1 / 5
SEC61B Antibody (PA3-015) in ICC/IF
Immunofluorescence analysis of SEC61B was performed using 70% confluent log phase MDCK cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SEC61B Rabbit Polyclonal Antibody (Product # PA3-015) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
PA3-015
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Pig,
Rat
Published species
Human,
Pig,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
whole serum
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA3-015 detects human, canine and porcine, and rat sec61 beta from microsomes and shows no cross-reactivity with other sec61 isoforms.
PA3-015 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~14 kDa protein representing sec61 beta from pig microsomes.
The PA3-015 immunogen is a synthetic peptide corresponding to residues M(1) P G P T P S G T N V G S S G R S P S K(20) of canine sec61 beta. This sequence is completely conserved between human, mouse, and canine.
Target Information
The Sec61 complex is responsible for translocation of proteins across the rough endoplasmic reticulum membrane. Sec61 is composed of three subunits: alpha, beta, and gamma; that associate to form a heterotrimer. Multiple heterotrimers associate forming the ER translocon. Sec61 alpha is a polytopic protein that has ten membrane-spanning loops. Sec61 has been found to be tightly associated with membrane bound ribosomes, either directly or through adaptor proteins, and is required for assembly of membrane and secretory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: protein translocation complex beta; protein translocation complex beta subunit; protein transport protein SEC61 beta subunit; Protein transport protein Sec61 subunit beta; sec61 beta; Sec61 beta subunit; Sec61 complex, beta subunit; SEC61 translocon beta subunit; SEC61B
Gene Aliases: 1190006C12Rik; AI326121; AW122942; SEC61B
UniProt ID: (Human) P60468, (Dog) P60467, (Mouse) Q9CQS8
Entrez Gene ID: (Human) 10952, (Dog) 404018, (Pig) 100511362, (Mouse) 66212, (Rat) 298068
SRP-dependent cotranslational protein targeting to membrane, translocation
intracellular protein transport
protein transport
retrograde protein transport, ER to cytosol
posttranslational protein targeting to membrane, translocation
ERAD pathway
ER-associated ubiquitin-dependent protein catabolic process
protein import into nucleus, translocation
transport
biological_process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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