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          • Primary Antibodies ›
          • Endothelin 1 Antibodies

          Invitrogen

          Endothelin 1 Polyclonal Antibody

          8 References
          View all (16) Endothelin 1 antibodies

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          Cite Endothelin 1 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          Endothelin 1 Antibody (PA3-067) in ICC/IF

          Immunofluorescence analysis of Endothelin 1 was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Endothelin 1 Rabbit Polyclonal Antibody (Product # PA3-067) at 1:250 dilution in 0.1% BSA and incubated for 3 ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Endothelin 1 Antibody in Immunocytochemistry (ICC/IF)
          Endothelin 1 Antibody in Immunohistochemistry (IHC)
          Endothelin 1 Antibody in Western Blot (WB)
          Endothelin 1 Polyclonal Antibody

          Product Details

          PA3-067

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:3,000-1:5,000
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 6 publications 6 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:2,000
          -

          Immunocytochemistry (ICC/IF)

          1:250
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Mouse Endothelin-1 CS-Abu-SSLMDKE-Abu-VYF-Abu-HLDIIW coupled to KLH.
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          whole serum

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2540494

          Product Specific Information

          PA3-067 detects Endothelin 1 from human, mouse, and rat samples.



          PA3-067 has been successfully used in Western blot, immunocytochemistry, and immunohistochemistry procedures. By Western blot, this antibody detects a 24.4 kDa protein corresponding to mouse Endothelin 1.



          The PA3-067 antigen is mouse Endothelin-1 CS-Abu-SSLMDKE-Abu-VYF-Abu-HLDIIW coupled to KLH.

          Target Information

          The protein encoded by this gene is proteolytically processed to release a secreted peptide termed endothelin 1. This peptide is a potent vasoconstrictor and is produced by vascular endothelial cells. Endothelin 1 also can affect the central nervous system. Two transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: Big endothelin 1; Big ET; Big ET 1; Big ET1; BigET; EDN1; Endothelin-1; Et; ET-1; PPET1; preproendothelin; Preproendothelin-1; unnamed protein product

          View more View less

          Gene Aliases: ARCND3; EDN1; ET-1; ET1; HDLCQ7; PPET1; preproET; QME

          View more View less

          UniProt ID: (Human) P05305, (Rat) P22388, (Mouse) P22387

          View more View less

          Entrez Gene ID: (Human) 1906, (Rat) 24323, (Mouse) 13614

          View more View less

          Function(s)
          receptor binding cytokine activity hormone activity protein binding endothelin A receptor binding endothelin B receptor binding receptor agonist activity peptide hormone
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter MAPK cascade skeletal system development patterning of blood vessels response to hypoxia in utero embryonic development histamine secretion response to amphetamine heart process glomerular filtration regulation of systemic arterial blood pressure by endothelin cardiac neural crest cell migration involved in outflow tract morphogenesis noradrenergic neuron differentiation transcription from RNA polymerase II promoter cellular calcium ion homeostasis regulation of pH smooth muscle contraction mitochondrion organization cell surface receptor signaling pathway G-protein coupled receptor signaling pathway adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway phospholipase C-activating G-protein coupled receptor signaling pathway positive regulation of cytosolic calcium ion concentration cell-cell signaling axon guidance heart development respiratory gaseous exchange body fluid secretion regulation of blood pressure positive regulation of cell proliferation response to xenobiotic stimulus dorsal/ventral pattern formation response to ozone positive regulation of heart rate gene expression positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy negative regulation of gene expression regulation of glucose transport neural crest cell development neural crest cell differentiation neural crest cell fate commitment response to activity artery smooth muscle contraction vein smooth muscle contraction regulation of vasoconstriction calcium-mediated signaling signal transduction involved in regulation of gene expression peptide hormone secretion nitric oxide transport negative regulation of blood coagulation positive regulation of cell migration neutrophil chemotaxis thyroid gland development neuron projection development positive regulation of prostaglandin biosynthetic process phospholipase D-activating G-protein coupled receptor signaling pathway positive regulation of prostaglandin secretion response to lipopolysaccharide response to testosterone negative regulation of smooth muscle cell apoptotic process response to prostaglandin F embryonic heart tube development response to nicotine intracellular signal transduction positive regulation of urine volume positive regulation of renal sodium excretion response to muscle stretch epithelial fluid transport vasoconstriction middle ear morphogenesis positive regulation of odontogenesis superoxide anion generation positive regulation of I-kappaB kinase/NF-kappaB signaling rhythmic excitation response to amino acid positive regulation of MAPK cascade protein kinase B signaling response to leptin cellular response to human chorionic gonadotropin stimulus leukocyte activation positive regulation of nitric oxide biosynthetic process positive regulation of cell size positive regulation of mitotic nuclear division positive regulation of transcription from RNA polymerase II promoter positive regulation of smooth muscle contraction positive regulation of JNK cascade positive regulation of hormone secretion negative regulation of hormone secretion blood vessel morphogenesis positive regulation of smooth muscle cell proliferation axon extension positive regulation of calcium-mediated signaling cartilage development negative regulation of protein metabolic process membrane depolarization canonical Wnt signaling pathway maternal process involved in parturition positive regulation of sarcomere organization axonogenesis involved in innervation positive regulation of cell growth involved in cardiac muscle cell development pharyngeal arch artery morphogenesis positive regulation of chemokine-mediated signaling pathway renal sodium ion absorption cellular response to hydrogen peroxide ERK1 and ERK2 cascade calcium ion transmembrane transport cellular response to calcium ion cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to follicle-stimulating hormone stimulus cellular response to luteinizing hormone stimulus cellular response to peptide hormone stimulus cellular response to glucocorticoid stimulus cellular response to mineralocorticoid stimulus cellular response to fatty acid cellular response to hypoxia cellular response to xenobiotic stimulus response to dexamethasone response to transforming growth factor beta cellular response to transforming growth factor beta stimulus glomerular endothelium development glomerular visceral epithelial cell differentiation endothelin receptor signaling pathway endothelin receptor signaling pathway involved in heart process positive regulation of neutrophil chemotaxis cellular response to toxic substance sympathetic neuron axon guidance regulation of blood vessel diameter cAMP/PKA signal transduction negative regulation of phospholipase C/protein kinase C signal transduction positive regulation of protein localization to nucleus positive regulation of NIK/NF-kappaB signaling response to salt semaphorin-plexin signaling pathway involved in axon guidance meiotic cell cycle process involved in oocyte maturation positive regulation of vascular smooth muscle cell proliferation positive regulation of artery morphogenesis prostaglandin biosynthetic process protein kinase C-activating G-protein coupled receptor signaling pathway multicellular organism aging positive regulation of receptor biosynthetic process phosphatidylinositol 3-kinase signaling glucose transport cell growth sensory perception of pain negative regulation of cAMP biosynthetic process negative regulation of cellular protein metabolic process cellular response to drug protein kinase C deactivation response to drug positive regulation of MAP kinase activity positive regulation of JUN kinase activity inositol phosphate-mediated signaling positive regulation of cytosolic calcium ion concentration involved in phospholipase C-activating G-protein coupled signaling pathway negative regulation of nitric-oxide synthase biosynthetic process regulation of sensory perception of pain positive regulation of prostaglandin-endoperoxide synthase activity regulation of blood vessel size
          It has to be done as per old AB suggested Products section.

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