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Invitrogen
beta Actin Polyclonal Antibody
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FIGURE: 1 / 16
beta Actin Antibody (PA5-16914) in ICC/IF
Immunofluorescence analysis of beta Actin was performed using 70% confluent log phase MDA-MB-231 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Actin Rabbit polyclonal antibody (Product # PA5-16914) at 2 µg/mL in 0.1% BSA and incubated for 3 hou... View More
Product Details
PA5-16914
Applications
Tested Dilution
Publications
Product Specifications
Published species
Dog,
Fruit fly,
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to N-terminus of human beta-actin
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
250 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA5-16914 targets Actin Beta in IHC (P) and WB applications and shows reactivity with Bovine, Canine, Chicken, Drosophila, Feline, Guinea Pig, Human, Leech, mouse, Ovine, Porcine, Rabbit, and Rat samples.
The PA5-16914 immunogen is synthetic peptide corresponding to N-terminus of human beta-actin.
Target Information
Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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