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Invitrogen
CXCR5 Polyclonal Antibody
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FIGURE: 1 / 2
CXCR5 Antibody (PA5-100726) in IHC (P)
Immunohistochemistry analysis of CXCR5 in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with CXCR5 polyclonal antibody (Product # PA5-100726) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-100726
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CXCR5(Accession P32302), corresponding to amino acid residues P4-V54.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total CXCR5.
Target Information
CD185, also known as CXCR5 or Burkitt lymphoma receptor 1 (BLR1), is a seven-transmembrane G protein-coupled receptor in the CXC chemokine receptor family. It plays a crucial role in lymphocyte trafficking, particularly in T cell migration into B cell follicles of germinal centers in response to CXCL13, making it a key marker of follicular helper T cells. In peripheral blood, CXCR5 is expressed on B cells, CD4+ T cells (excluding Th1 and Th2 cells), and a subset of memory (CD45RO+) T cells. These CD185+ T cells are in a resting state and migrate to lymph nodes due to CCR7 and CD62L expression. In tonsils, CD185 is found on nearly all CD4+ cells, along with activation markers like CD69 and ICOS, and can induce antibody production when co-cultured with B cells. CXCR5 is expressed in lymphatic tissues such as lymph node follicles and the spleen, and is also found in human blood and salivary glands, as well as various animal tissues. It is essential for B cell migration and is part of the larger family of G protein-coupled receptors (GPCRs), which have seven membrane-spanning helices and are involved in numerous signaling pathways. GPCRs, including CXCR5, are regulated by various ligands that affect their conformation and signaling through G-proteins and downstream effectors. The regulation of GPCRs, including their deactivation, has significant implications for cellular functions and is a major focus of pharmacological research, leading to the development of drugs like Zyprexa and Claritin for mental health and allergy treatments.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Blr-1; Burkitt lymphoma receptor 1; Burkitt lymphoma receptor 1 homolog; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); Burkitt lymphoma receptor 1, GTP-binding protein; C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 5; CC motif chemokine; CCmotif chemokine; CD185; CD185 antigen; chemochine (C-X-C motif) receptor 5; chemokine (C-X-C motif) receptor 5; CXC; CXC motif chemokine; CXC-R5; CXCR-5; Fusin; GPR9; HUMSTR; LESTR; MDR-15; Monocyte-derived receptor 15; Neurolymphatic receptor; NLR; unnamed protein product
Gene Aliases: BLR1; CD185; CXC-R5; CXCR-5; CXCR5; Gpcr6; MDR15; NLR
UniProt ID: (Human) P32302, (Rat) P34997, (Mouse) Q04683
Entrez Gene ID: (Human) 643, (Rat) 29363, (Mouse) 12145
chemotaxis
immune response
G-protein coupled receptor signaling pathway
positive regulation of cytokinesis
B cell activation
lymph node development
chemokine-mediated signaling pathway
signal transduction
positive regulation of cytosolic calcium ion concentration
calcium-mediated signaling
leukocyte chemotaxis
cell chemotaxis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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