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Invitrogen
GNAI3 Polyclonal Antibody
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FIGURE: 1 / 3
GNAI3 Antibody (PA5-101624) in ICC/IF
Immunocytochemistry analysis of GNAI3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with GNAI3 polyclonal antibody (Product # PA5-101624) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101624
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human GNAI3(Accession P08754), corresponding to amino acid residues L39-G89.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total GNAI3.
Target Information
Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (a photon, pheromone, odorant, hormone or neurotransmitter) while the effectors (i. e. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein alpha, beta and gamma polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their alpha subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Four distinct classes of Galpha subunits have been identified; these include Gs, Gi, Gq and Galpha 12/13. The Gi class comprises all the known alpha subunits that are susceptible to pertussis toxin modifications, including Galpha i-1, Galpha i-2, Galpha i-3, Galpha o, Galpha t1, Galpha t2, Galpha z and Galpha gust. Of these, the three Galpha i subtypes function to open atrial potassium channels.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: G protein; G protein alpha subunit; G(i) alpha-3; GTP-binding protein alpha subunit; guanine nucleotide binding protein (G protein), alpha inhibiting 3; guanine nucleotide binding protein (G protein), alpha inhibiting activity polypeptide 3; guanine nucleotide binding protein, alpha inhibiting 3; Guanine nucleotide binding, protein, alpha inhibiting polypeptide 3; guanine nucleotide-binding protein (G) alpha subunit; guanine nucleotide-binding protein G(i) subunit alpha; Guanine nucleotide-binding protein G(i) subunit alpha-3; guanine nucleotide-binding protein G(k) subunit alpha; guanine nucleotide-binding regulatory protein alpha-inhibitory subunit; heterotrimeric guanine nucleotide-binding protein 1A; unnamed protein product
Gene Aliases: 87U6; AI158965; ARCND1; ARCODS; AW537698; Galphai3; GIP3A; Gnai-3; GNAI3; HG1A
UniProt ID: (Human) P08754, (Rat) P08753, (Mouse) Q9DC51
Entrez Gene ID: (Human) 2773, (Rat) 25643, (Mouse) 14679
nucleotide binding
G-protein coupled receptor binding
GTPase activity
protein binding
GTP binding
guanyl nucleotide binding
GDP binding
G-protein beta/gamma-subunit complex binding
metal ion binding
signal transducer activity
protein domain specific binding
G-protein coupled serotonin receptor binding
GTPase activating protein binding
heterotrimeric G-protein
signal transduction
G-protein coupled receptor signaling pathway
adenylate cyclase-modulating G-protein coupled receptor signaling pathway
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
negative regulation of adenylate cyclase activity
positive regulation of macroautophagy
GTP metabolic process
cell division
vesicle fusion
regulation of heart contraction
metabolic process
negative regulation of secretion
cell cycle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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