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Invitrogen
NSG1 Polyclonal Antibody
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FIGURE: 1 / 2
NSG1 Antibody (PA5-101719) in ICC/IF
Immunocytochemistry analysis of NSG1 in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with NSG1 polyclonal antibody (Product # PA5-101719) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101719
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NSG1(Accession P42857), corresponding to amino acid residues A111-V161.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total NSG1.
Target Information
NEEP21 (neuron-enriched endosomal 21kDa protein), also known as brain neuron cytoplasmic protein 1, NSG1 (neuron-specific proteins family member 1), P21 or D4S234E, is a single pass type II membrane protein belonging to the NSG family. It is highly expressed during neuronal maturation but its expression is downregulated in adult tissues. NEEP21 predominantly localizes to Rab 4-positive early endosomes in the somatodendritic neuronal compartment and is essential for proper receptor sorting and recycling in neurons. It associates with GRIP1 and GluR-2 and mediates the surface expression of GluR-2. When this interaction is interrupted, GluR-2 accumulates in early endosomes and leads to changes in evoked synaptic current properties. In addition, NEEP21 forms a complex with the SNARE protein, Syntaxin 13 (also known as Syntaxin 12), and participates in the recycling of transferrin receptors (TFRs) and NTR2 (Neurotensin receptor 2).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: brain neuron cytoplasmic protein 1; Brain neuron cytoplasmic protein 1/2; brain specific mRNA b; carboxyterminally EE-tagged neuron-enriched endosomal 21 kDa protein; M234; neuron specific gene family member 1; Neuron-enriched endosomal protein of 21 kDa; Neuron-specific protein family member 1; Neuronal vesicle trafficking-associated protein 1; p21; unnamed protein product
Gene Aliases: Bsmrb; D4S234; D4S234E; m234; NEEP21; NSG1; P21; PEP1
UniProt ID: (Human) P42857, (Mouse) Q62092, (Rat) P02683
Entrez Gene ID: (Human) 27065, (Mouse) 18196, (Rat) 25247
receptor recycling
positive regulation of receptor recycling
apoptotic process
endosomal transport
amyloid precursor protein metabolic process
clathrin coat assembly
spontaneous synaptic transmission
neurotransmitter receptor transport, endosome to postsynaptic membrane
vesicle-mediated transport in synapse
neurotransmitter receptor cycle
postsynaptic neurotransmitter receptor cycle
regulation of long-term synaptic potentiation
dopamine receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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