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Invitrogen
RRAGC Polyclonal Antibody
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FIGURE: 1 / 3
RRAGC Antibody (PA5-101820) in ICC/IF
Immunocytochemistry analysis of RRAGC in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with RRAGC polyclonal antibody (Product # PA5-101820) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101820
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human RRAGC(Accession Q9HB90), corresponding to amino acid residues S281-V331.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total RRAGC.
Target Information
RRAGC (Ras-related GTP-binding protein C) is a monomeric guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulations of the mTORC1 signaling cascade. RRAGC forms a heterodimer with RRAGA and RRAGB and is primarily localized to the cytoplasm. It cycles between an inactive GTP-bound and an active GDP-bound form. RRAGC, in its active form, participates in the re-localization of mTORC1 to the lysosomes and its subsequent activation by the GTPase RHEB. This re-localization is a crucial step in the activation of the TOR signaling cascade by amino acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: GIP2; GTPase-interacting protein 2; putative Ras-like GTP-binding protein; Rag C; Rag C protein; Ras-related GTP-binding protein C; rragc; small GTPase homolog; small GTPase, homolog; TIB929; unnamed protein product
Gene Aliases: AU041672; GTR2; LNGODS; LNGOS; RAGC; RRAGC; TIB929; YGR163W
UniProt ID: (Human) Q9HB90, (Mouse) Q99K70
Entrez Gene ID: (Human) 64121, (Mouse) 54170, (Rat) 298514
autophagosome assembly
cytoplasmic translation
transcription, DNA-templated
apoptotic process
lysosome organization
small GTPase mediated signal transduction
protein localization
RNA splicing
cellular response to starvation
negative regulation of autophagy
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
protein destabilization
cellular response to nutrient levels
regulation of TOR signaling
cellular response to amino acid starvation
TORC1 signaling
response to amino acid
negative regulation of translational initiation
positive regulation of translational initiation
protein localization to lysosome
cellular response to amino acid stimulus
protein localization to membrane
negative regulation of protein localization to nucleus
regulation of TORC1 signaling
positive regulation of TORC1 signaling
regulation of autophagy
positive regulation of TOR signaling
biological_process
metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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