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Invitrogen
POLG Polyclonal Antibody
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FIGURE: 1 / 4
POLG Antibody (PA5-103791) in IHC (P)
Immunohistochemistry analysis of POLG in mouse kidney tissue. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with POLG polyclonal antibody (Product # PA5-103791) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-103791
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human POLG(Accession P54098), corresponding to amino acid residues Y452-K502.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total POLG1.
Target Information
Mitochondrial DNA polymerase is heterotrimeric, consisting of a homodimer of accessory subunits plus a catalytic subunit. The protein encoded by this gene is the catalytic subunit of mitochondrial DNA polymerase. The encoded protein contains a polyglutamine tract near its N-terminus that may be polymorphic. Defects in this gene are a cause of progressive external ophthalmoplegia with mitochondrial DNA deletions 1 (PEOA1), sensory ataxic neuropathy dysarthria and ophthalmoparesis (SANDO), Alpers-Huttenlocher syndrome (AHS), and mitochondrial neurogastrointestinal encephalopathy syndrome (MNGIE). Two transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 3'-5' exodeoxyribonuclease; 5'-deoxyribose-phosphate lyase; DNA polymerase gamma; DNA polymerase gamma subunit 1 (Mitochondrial DNA polymerase catalytic subunit) (PolG-alpha); DNA polymerase subunit gamma-1; DNA-directed DNA polymerase gamma; FLJ27114; Mitochondrial DNA polymerase catalytic subunit; mitochondrial DNA polymerase gamma; mitochondrial DNA polymerase-gamma; mitochondrial polymerase gamma; mitochondrial polymerase gamma catalytic subunit; Pol gamma; PolG-alpha; polymerase (DNA directed), gamma; polymerase (DNA) gamma, catalytic subunit; polymerase, gamma; unnamed protein product
Gene Aliases: AA409516; MDP1; Mip1; MIRAS; MTDPS4A; MTDPS4B; PEO; POLG; POLG1; POLGA; SANDO; SCAE
UniProt ID: (Human) P54098, (Rat) Q9QYV8
Entrez Gene ID: (Human) 5428, (Mouse) 18975, (Rat) 85472
protease binding
DNA binding
chromatin binding
DNA-directed DNA polymerase activity
protein binding
single-stranded DNA 3'-5' exodeoxyribonuclease activity
3'-5' exonuclease activity
transferase activity
nucleotidyltransferase activity
hydrolase activity
lyase activity
DNA polymerase activity
5'-deoxyribose-5-phosphate lyase activity
catalytic activity, acting on a nucleic acid
nucleic acid binding
exonuclease activity
DNA-directed DNA polymerase
DNA metabolic process
DNA replication
DNA-dependent DNA replication
mitochondrial DNA replication
DNA repair
base-excision repair
base-excision repair, gap-filling
DNA replication proofreading
DNA biosynthetic process
aging
nucleic acid phosphodiester bond hydrolysis
response to light stimulus
response to gamma radiation
response to hyperoxia
cellular response to glucose stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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