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          • Primary Antibodies ›
          • SMO Antibodies

          Invitrogen

          SMO Polyclonal Antibody

          View all (27) SMO antibodies

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          Cite SMO Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (5)
          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          FIGURE: 1 / 5

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          SMO Antibody (PA5-104390) in IHC (P)

          Immunohistochemistry analysis of SMO in human colorectal cancer. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with SMO polyclonal antibody (Product # PA5-104390) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMO Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          SMO Antibody in Western Blot (WB)
          SMO Polyclonal Antibody

          Product Details

          PA5-104390

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          1:50-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:500
          -
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          A synthesized peptide derived from human SMO(Accession Q99835), corresponding to amino acid residues N76-L126.
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS, pH 7.4, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2853697

          Product Specific Information

          Antibody detects endogenous levels of total SMO.

          Target Information

          Smoothened drosophila homolog (SMOH) is a serpentine protein belonging to the G-protein coupled receptor Fz/Smo family with 787 aa, seven transmembrane G protein-coupled receptors with 4 glycosylation sites and a putative extracellular amino terminus. Smoothened activated by b-Arrestin 2 and GRK2 acts as a receptor for Shh, the Sonic hedgehog gene product, and is a positive regulator of hedgehog signaling pathway along with the smoothened inhibitor patched protein (PTCH) and regulates vertebrate developmental processes, cellular proliferation and differentiation by activating transcription factor Gli. Smoothened mutants are known to cause sporadic basal cell carcinoma of the skin. It is known to express widely in heart and brain.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: bent body; candidate receptor for sonic hedgehog; frizzled family member 11; HGNC:11119; Protein Gx; Protein SMOH; Protein smoothened; seven transmembrane helix receptor; smoothened homolog; smoothened, frizzled family receptor; smoothened, seven transmembrane spanning receptor

          View more View less

          Gene Aliases: bnb; CRJS; E130215L21Rik; FZD11; Gx; PHLS; SMO; SMOH; smoothened

          View more View less

          UniProt ID: (Human) Q99835, (Mouse) P56726, (Rat) P97698

          View more View less

          Entrez Gene ID: (Human) 6608, (Mouse) 319757, (Rat) 25273

          View more View less

          Function(s)
          cAMP-dependent protein kinase inhibitor activity transmembrane signaling receptor activity G-protein coupled receptor activity patched binding protein binding oxysterol binding protein kinase A catalytic subunit binding protein sequestering activity signal transducer activity drug binding Wnt-protein binding molecular_function Wnt-activated receptor activity transmembrane signal receptor
          Process(es)
          negative regulation of transcription from RNA polymerase II promoter ossification vasculogenesis osteoblast differentiation in utero embryonic development cell fate specification neural crest cell migration negative regulation of protein phosphorylation hair follicle development heart looping positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation heart morphogenesis determination of left/right asymmetry in lateral mesoderm type B pancreatic cell development protein import into nucleus apoptotic process signal transduction cell surface receptor signaling pathway G-protein coupled receptor signaling pathway smoothened signaling pathway determination of left/right symmetry ventral midline determination pattern specification process neuroblast proliferation central nervous system development midgut development cell proliferation positive regulation of cell proliferation tissue development anterior/posterior pattern specification dorsal/ventral pattern formation gene expression regulation of gene expression positive regulation of gene expression negative regulation of gene expression spinal cord dorsal/ventral patterning dentate gyrus development cerebellar cortex morphogenesis thalamus development dorsal/ventral neural tube patterning central nervous system neuron differentiation cerebral cortex development positive regulation of cell migration epithelial cell differentiation negative regulation of epithelial cell differentiation hair follicle morphogenesis protein localization to nucleus multicellular organism growth positive regulation of multicellular organism growth positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth negative regulation of apoptotic process negative regulation of DNA binding positive regulation of smoothened signaling pathway negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of organ growth astrocyte activation cell development digestive tract development embryonic organ development developmental growth skeletal muscle fiber development smooth muscle tissue development forebrain morphogenesis homeostasis of number of cells within a tissue epithelial cell proliferation positive regulation of epithelial cell proliferation protein stabilization myoblast migration negative regulation of hair follicle development contact inhibition atrial septum morphogenesis mammary gland epithelial cell differentiation epithelial-mesenchymal cell signaling somite development pancreas morphogenesis left/right axis specification cellular response to cholesterol dopaminergic neuron differentiation commissural neuron axon guidance mesenchymal to epithelial transition involved in metanephric renal vesicle formation positive regulation of branching involved in ureteric bud morphogenesis response to inositol regulation of somatic stem cell population maintenance regulation of heart morphogenesis positive regulation of hh target transcription factor activity multicellular organism development smoothened signaling pathway involved in ventral spinal cord patterning smoothened signaling pathway involved in regulation of cerebellar granule cell precursor cell proliferation axon extension involved in axon guidance detection of cell density by contact stimulus involved in contact inhibition regulation of stem cell population maintenance spermatogenesis response to wounding response to organic substance facial nerve development neuron projection regeneration osteoblast proliferation non-canonical Wnt signaling pathway canonical Wnt signaling pathway renal system development
          It has to be done as per old AB suggested Products section.

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