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Invitrogen
Phospho-IkappaB epsilon (Ser161) Polyclonal Antibody
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FIGURE: 1 / 10
Phospho-IkappaB epsilon (Ser161) Antibody (PA5-104688) in IHC (P)
Immunohistochemistry analysis of paraffin-embedded Phospho-IkappaB epsilon (Ser22) in human appendiceal tissue sections. Antigen retrieval was performed using citrate buffer. Samples were blocked with blocking buffer (1.5 hr, 22°C), incubated with Phospho-IkappaB epsilon (Ser22) polyclonal antibody (Product # PA5-104688) using a dilution of 1:100 (1.5 hr, 22°C), followed by HRP conjugated goat anti-rabbit.
Product Details
PA5-104688
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human NFKBIE(Accession O00221), corresponding to amino acid residues around phosphorylated Ser161.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
sequential chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of IKB epsilon only when phosphorylated at Serine 161.
Target Information
NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NF-kappa-B by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). For some genes, activation requires NFKB interaction with other transcription factors, such as STAT (see STAT6, MIM 601512), AP1 (JUN, MIM 165160), and NFAT (see NFATC1, MIM 600489).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: I-kappa-B-epsilon; ikappaBepsilon; IkB epsilon; IkB-E; ikB-epsilon; IkBepsilon; NF-kappa-B inhibitor epsilon; NF-kappa-BIE; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, epsilon; solute carrier family 35, member B2; unnamed protein product
Gene Aliases: I-kappa-B-epsilon; IkB-E; ikB-epsilon; IKBE; NF-kappa-BIE; NFKBIE; Slc35b2
UniProt ID: (Human) O00221, (Mouse) O54910
Entrez Gene ID: (Human) 4794, (Mouse) 18037, (Rat) 316241
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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