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Invitrogen

Phospho-IRS1 (Ser639) Polyclonal Antibody

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Phospho-IRS1 (Ser639) Polyclonal Antibody

Product Details

PA5-104877

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human IRS1(Accession P35568), corresponding to amino acid residues around phosphorylated Ser639.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

sequential chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2816350

Product Specific Information

Antibody detects endogenous levels of IRS1 only when phosphorylated at Serine 639.

Target Information

IRS-1, a major substrate of the insulin receptor, is phosphorylated in response to stimulation of cells by insulin, insulin-like growth factor 1 (IGF-1) and interleukin 4 (IL-4). IRS-1 is phosphorylated on serine, threonine and tyrosine residues in a variety of tissues. An insulin-sensitive serine/threonine kinase casein kinase II mediates a portion of the insulin-stimulated serine/threonine phosphorylation of overexpressed IRS-1 in vivo. Thr 502 is identified as the major casein kinase II-catalyzed phosphorylation site in rat IRS-1, and Ser 99 is an additional phosphorylation site catalyzed by casein kinase II. Thus, casein kinase II-catalyzed phosphorylation of IRS-1 may be a component of the intracellular insulin signaling cascade. IRS-1 contains three putative binding sites for 14-3-3 (Ser 270, Ser 374 and Ser 641) and the motif around Ser 270 is located in the phosphortyrosine binding domain of IRS-1, which is responsible for the interaction with the insulin receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Insulin receptor substrate 1; IRS-1; pp185

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Gene Aliases: G972R; HIRS-1; IRS-1; IRS1; IRS1IRM

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UniProt ID: (Human) P35568, (Rat) P35570, (Mouse) P35569

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Entrez Gene ID: (Human) 3667, (Rat) 25467, (Mouse) 16367

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Function(s)
phosphotyrosine binding transmembrane receptor protein tyrosine kinase adaptor activity protein kinase C binding receptor binding insulin receptor binding insulin-like growth factor receptor binding protein binding receptor signaling complex scaffold activity signaling adaptor activity SH2 domain binding phosphatidylinositol 3-kinase binding signal transducer activity protein kinase binding protein domain specific binding
Process(es)
signal transduction positive regulation of cell proliferation insulin receptor signaling pathway positive regulation of signal transduction positive regulation of glucose metabolic process cytokine-mediated signaling pathway positive regulation of fatty acid beta-oxidation response to insulin cellular response to insulin stimulus glucose homeostasis protein kinase B signaling positive regulation of glycogen biosynthetic process positive regulation of glucose import negative regulation of insulin receptor signaling pathway positive regulation of insulin receptor signaling pathway negative regulation of insulin secretion insulin-like growth factor receptor signaling pathway positive regulation of protein kinase B signaling cellular response to fatty acid positive regulation of mesenchymal cell proliferation regulation of gene expression phosphatidylinositol 3-kinase signaling lipid catabolic process insulin secretion positive regulation of cell migration mammary gland development response to caffeine protein localization to nucleus positive regulation of phosphorylation response to peptide hormone positive regulation of phosphatidylinositol 3-kinase activity protein heterooligomerization positive regulation of glucagon secretion negative regulation of somatostatin secretion positive regulation of glucose import in response to insulin stimulus
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